Inactivation of hepatitis B virus: Evaluation of the efficacy of the disinfectant 'Solprogel' using a DNA-polymerase activity assay

A. Hernández; F. J. Belda; J. Domínguez; L. Matas; M. Giménez; M. Caraballo; C. Ramil; V. Ausina

doi:10.1016/S0195-6701(97)90057-8

Inactivation of hepatitis B virus: Evaluation of the efficacy of the disinfectant 'Solprogel' using a DNA-polymerase activity assay

A. Hernández, F. J. Belda, J. Domínguez, L. Matas, M. Giménez, M. Caraballo, C. Ramil, V. Ausina

Department of Genetics and Microbiology

Research output: Contribution to journal › Article › Research › peer-review

10 Citations (Scopus)

Abstract

The effects of sodium dichloroisocyanurate (NaDCC) and Solprogel (Laboratories Inibsa, S.A., Barcelona, Spain), a compound that contains NaDCC plus a biodegradable polymer of acrylic acid, on the activity of DNA polymerase (DNA-P) associated with hepatitis B virus in serum were evaluated. DNA-P positive and negative pools of human serum samples were used as positive and negative stock virus. Inhibition of DNA-P activity by NaDCC and the commercial product was found to be concentration-dependent. Two minutes exposure to the minimum effective concentration of NaDCC (1000 ppm available chlorine) or Solprogel 16% (960 ppm available chlorine) totally inhibited DNA-P activity.

Original language	English
Pages (from-to)	305-312
Journal	Journal of Hospital Infection
Volume	36
Issue number	4
DOIs	https://doi.org/10.1016/S0195-6701(97)90057-8
Publication status	Published - 1 Jan 1997

Keywords

DNA-polymerase
Hepatitis B virus
Sodium dichloroisocyanurate
Solprogel

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title = "Inactivation of hepatitis B virus: Evaluation of the efficacy of the disinfectant 'Solprogel' using a DNA-polymerase activity assay",

abstract = "The effects of sodium dichloroisocyanurate (NaDCC) and Solprogel (Laboratories Inibsa, S.A., Barcelona, Spain), a compound that contains NaDCC plus a biodegradable polymer of acrylic acid, on the activity of DNA polymerase (DNA-P) associated with hepatitis B virus in serum were evaluated. DNA-P positive and negative pools of human serum samples were used as positive and negative stock virus. Inhibition of DNA-P activity by NaDCC and the commercial product was found to be concentration-dependent. Two minutes exposure to the minimum effective concentration of NaDCC (1000 ppm available chlorine) or Solprogel 16% (960 ppm available chlorine) totally inhibited DNA-P activity.",

keywords = "DNA-polymerase, Hepatitis B virus, Sodium dichloroisocyanurate, Solprogel",

author = "A. Hern{\'a}ndez and Belda, {F. J.} and J. Dom{\'i}nguez and L. Matas and M. Gim{\'e}nez and M. Caraballo and C. Ramil and V. Ausina",

year = "1997",

month = jan,

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doi = "10.1016/S0195-6701(97)90057-8",

language = "English",

volume = "36",

pages = "305--312",

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Inactivation of hepatitis B virus: Evaluation of the efficacy of the disinfectant 'Solprogel' using a DNA-polymerase activity assay. / Hernández, A.; Belda, F. J.; Domínguez, J.; Matas, L.; Giménez, M.; Caraballo, M.; Ramil, C.; Ausina, V.

In: Journal of Hospital Infection, Vol. 36, No. 4, 01.01.1997, p. 305-312.

Research output: Contribution to journal › Article › Research › peer-review

TY - JOUR

T1 - Inactivation of hepatitis B virus: Evaluation of the efficacy of the disinfectant 'Solprogel' using a DNA-polymerase activity assay

AU - Hernández, A.

AU - Belda, F. J.

AU - Domínguez, J.

AU - Matas, L.

AU - Giménez, M.

AU - Caraballo, M.

AU - Ramil, C.

AU - Ausina, V.

PY - 1997/1/1

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N2 - The effects of sodium dichloroisocyanurate (NaDCC) and Solprogel (Laboratories Inibsa, S.A., Barcelona, Spain), a compound that contains NaDCC plus a biodegradable polymer of acrylic acid, on the activity of DNA polymerase (DNA-P) associated with hepatitis B virus in serum were evaluated. DNA-P positive and negative pools of human serum samples were used as positive and negative stock virus. Inhibition of DNA-P activity by NaDCC and the commercial product was found to be concentration-dependent. Two minutes exposure to the minimum effective concentration of NaDCC (1000 ppm available chlorine) or Solprogel 16% (960 ppm available chlorine) totally inhibited DNA-P activity.

AB - The effects of sodium dichloroisocyanurate (NaDCC) and Solprogel (Laboratories Inibsa, S.A., Barcelona, Spain), a compound that contains NaDCC plus a biodegradable polymer of acrylic acid, on the activity of DNA polymerase (DNA-P) associated with hepatitis B virus in serum were evaluated. DNA-P positive and negative pools of human serum samples were used as positive and negative stock virus. Inhibition of DNA-P activity by NaDCC and the commercial product was found to be concentration-dependent. Two minutes exposure to the minimum effective concentration of NaDCC (1000 ppm available chlorine) or Solprogel 16% (960 ppm available chlorine) totally inhibited DNA-P activity.

KW - DNA-polymerase

KW - Hepatitis B virus

KW - Sodium dichloroisocyanurate

KW - Solprogel

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DO - 10.1016/S0195-6701(97)90057-8

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EP - 312

JO - Journal of Hospital Infection

JF - Journal of Hospital Infection

SN - 0195-6701

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