In Vivo, non-invasive characterization of human bone by hybrid broadband (600-1200 nm) diffuse optical and correlation spectroscopies

Sanathana Konugolu Venkata Sekar, Marco Pagliazzi, Eugènia Negredo, Fabrizio Martelli, Andrea Farina, Alberto Dalla Mora, Claus Lindner, Parisa Farzam, Núria Pérez-Álvarez, Jordi Puig, Paola Taroni, Antonio Pifferi, Turgut Durduran

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    © 2016 Konugolu Venkata Sekar et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Non-invasive in vivo diffuse optical characterization of human bone opens a new possibility of diagnosing bone related pathologies. We present an in vivo characterization performed on seventeen healthy subjects at six different superficial bone locations: radius distal, radius proximal, ulna distal, ulna proximal, trochanter and calcaneus. A tailored diffuse optical protocol for high penetration depth combined with the rather superficial nature of considered tissues ensured the effective probing of the bone tissue. Measurements were performed using a broadband system for Time-Resolved Diffuse Optical Spectroscopy (TRS) to assess mean absorption and reduced scattering spectra in the 600-1200 nm range and Diffuse Correlation Spectroscopy (DCS) to monitor microvascular blood flow. Significant variations among tissue constituents were found between different locations; with radius distal rich of collagen, suggesting it as a prominent location for bone related measurements, and calcaneus bone having highest blood flow among the body locations being considered. By using TRS and DCS together, we are able to probe the perfusion and oxygen consumption of the tissue without any contrast agents. Therefore, we predict that these methods will be able to evaluate the impairment of the oxygen metabolism of the bone at the point-of-care.
    Original languageEnglish
    Article numbere0168426
    JournalPLoS ONE
    Issue number12
    Publication statusPublished - 1 Dec 2016


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