In vivo genotoxic effects of four different nano-sizes forms of silica nanoparticles in Drosophila melanogaster

Eşref Demir, Sezgin Aksakal, Fatma Turna, Bülent Kaya, Ricard Marcos

Research output: Contribution to journalArticleResearchpeer-review

26 Citations (Scopus)

Abstract

© 2014 Elsevier B.V. All rights reserved. Although the use of synthetic amorphous silica (SAS) is steady increasing, scarce information exists on its potential health risk. In particular few and conflictive data exist on its genotoxicity. To fill in this gap we have used Drosophila melanogaster as in vivo model test organism to detect the genotoxic activity of different SAS with different primary sizes (6, 15, 30 and 55nm). The wing-spot assay and the comet assay in larvae haemocytes were used, and the obtained results were compared with those obtained with the microparticulated form (silicon dioxide). All compounds were administered to third instar larvae at concentrations ranging from 0.1 to 10mM. No significant increases in the frequencies of mutant spots were observed in the wing-spot assay with any of the tested compounds. On the other hand, significant dose-dependent increases in the levels of primary DNA damage, measured by the comet assay, were observed for all the SAS evaluated but mainly when high doses (5 and 10mM) were used. These in vivo results contribute to increase the database dealing with the potential genotoxic risk associated to SAS nanoparticles exposure.
Original languageEnglish
Pages (from-to)260-266
JournalJournal of Hazardous Materials
Volume283
DOIs
Publication statusPublished - 1 Jan 2015

Keywords

  • Alkaline comet assay
  • Drosophila melanogaster
  • Genotoxicity
  • SAS nanoparticles
  • Wing spot assay

Fingerprint Dive into the research topics of 'In vivo genotoxic effects of four different nano-sizes forms of silica nanoparticles in Drosophila melanogaster'. Together they form a unique fingerprint.

Cite this