In vivo dynamic metabolic changes after transplantation of induced pluripotent stem cells for ischemic injury

Shuang Wu, Yuankai Zhu, Hao Liu, Ling Tang, Ruili Du, Yehua Shen, Jin Feng, Kai Zhang, Caiyun Xu, Shouhong Zhang, Yao Chen, Fahuan Song, Yunqi Zhu, Weizhong Gu, Ping Liang, Ignasi Carrió, Hong Zhang, Mei Tian

Research output: Contribution to journalArticleResearchpeer-review

5 Citations (Scopus)

Abstract

COPYRIGHT © 2016 by the Society of Nuclear Medicine and Molecular Imaging, Inc. This study aimed to investigate in vivo dynamic metabolic changes after transplantation of induced pluripotent stem cells (iPSCs) and iPSC-derived enriched cardiomyocytes (iPSC-CMs) in a rat model of ischemic injury. Methods: Serial 18F-FDG PET, echocardiographic, immunohistochemical, and immunofluorescence studies were performed after transplantation of iPSCs and iPSC-CMs and compared with embryonic stem cells (ESCs), ESC-CMs, and a phosphatebuffered saline control group of rats with myocardial infarction. Results: Increased glucose metabolism in periinfarct areas and improved myocardial function were observed in the stem cell transplantation groups compared with the control group, and serial immunofluorescence and immunohistochemical results exhibited the survival and migration of stem cells during the study period. Conclusion: Serial 18F-FDG PET and echocardiographic imaging studies demonstrated the dynamic metabolic changes and recovery of myocardial function after stem cell transplantation. 18F-FDG PET could be a potential approach to evaluating spatiotemporal dynamic metabolic changes in vivo after transplantation of iPSCs or iPSC-CMs for ischemic injury.
Original languageEnglish
Pages (from-to)2012-2015
JournalJournal of Nuclear Medicine
Volume57
Issue number12
DOIs
Publication statusPublished - 1 Dec 2016

Keywords

  • Induced pluripotent stem cell (iPSC)
  • Induced PSC-derived cardiomyocytes (iPSC-CMs)
  • Metabolism
  • Myocardial ischemia
  • Positron emission tomography (PET)

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