In vivo and ex vivo magnetic resonance spectroscopy of the infarct and the subventricular zone in experimental stroke

Elena Jiménez-Xarrié, Myriam Davila, Sara Gil-Perotín, Andrés Jurado-Rodríguez, Ana Paula Candiota, Raquel Delgado-Mederos, Silvia Lope-Piedrafita, José Manuel García-Verdugo, Carles Arús, Joan Martí-Fàbregas

Research output: Contribution to journalArticleResearchpeer-review

14 Citations (Scopus)

Abstract

© 2015 ISCBFM. Ex vivo high-resolution magic-angle spinning (HRMAS) provides metabolic information with higher sensitivity and spectral resolution than in vivo magnetic resonance spectroscopy (MRS). Therefore, we used both techniques to better characterize the metabolic pattern of the infarct and the neural progenitor cells (NPCs) in the ipsilateral subventricular zone (SVZi). Ischemic stroke rats were divided into three groups: G0 (non-stroke controls, n=6), G1 (day 1 after stroke, n=6), and G7 (days 6 to 8 after stroke, n=12). All the rats underwent MRS. Three rats per group were analyzed by HRMAS. The remaining rats were used for immunohistochemical studies. In the infarct, both techniques detected significant metabolic changes. The most relevant change was in mobile lipids (2.80 ppm) in the G7 group (a 5.53- and a 3.95-fold increase by MRS and HRMAS, respectively). In the SVZi, MRS did not detect any significant metabolic change. However, HRMAS detected a 2.70-fold increase in lactate and a 0.68-fold decrease in N-acetylaspartate in the G1 group. None of the metabolites correlated with the 1.37-fold increase in NPCs detected by immunohistochemistry in the G7 group. In conclusion, HRMAS improves the metabolic characterization of the brain in experimental ischemic stroke. However, none of the metabolites qualifies as a surrogate biomarker of NPCs.
Original languageEnglish
Pages (from-to)828-834
JournalJournal of Cerebral Blood Flow and Metabolism
Volume35
DOIs
Publication statusPublished - 1 Jan 2015

Keywords

  • HRMAS
  • MRS
  • neurogenesis
  • spectroscopy
  • stroke

Fingerprint

Dive into the research topics of 'In vivo and ex vivo magnetic resonance spectroscopy of the infarct and the subventricular zone in experimental stroke'. Together they form a unique fingerprint.

Cite this