TY - JOUR
T1 - In vitro toxicological assessment of an organosulfur compound from Allium extract: Cytotoxicity, mutagenicity and genotoxicity studies
AU - Mellado-García, Pilar
AU - Maisanaba, Sara
AU - Puerto, María
AU - Prieto, Ana Isabel
AU - Marcos, Ricard
AU - Pichardo, Silvia
AU - Cameán, Ana María
PY - 2017/1/1
Y1 - 2017/1/1
N2 - © 2016 Elsevier Ltd Garlic (Allium sativum) and onion (Allium cepa) are being used in the food industry as flavoring but also for their antimicrobial activities. These activities are mainly derived from the organosulfur compounds (OSCs). Propyl propane thiosulfinate (PTS) is an OSC with potential use in the active packaging, but its safety should be guaranteed before being commercialized. The aim of this work was to investigate for the first time the cytotoxicity of PTS as well as its in vitro mutagenic/genotoxic potential using the following battery of genotoxicity tests:(1)the bacterial reverse-mutation assay in S. typhimurium (Ames test, OECD 471, 1997); (2) the micronucleus test (MN, OECD 487, 2016); (3) the mouse lymphoma thymidine-kinase assay (MLA, OECD 476, 2015), and (4) the comet assay (standard and modified with restriction enzymes). The results revealed that PTS was not mutagenic neither in the Ames test nor in MLA. However, genotoxic effects were recorded in the MN test on mammalian cells (L5178YTk+/−cells) after PTS exposure at the highest concentration tested (17.25 μM) without S9, and also its metabolites (+S9, from 20 μM). Moreover, in the comet assay, PTS induced DNA breaks damage in Caco-2 cells at the highest concentration tested (280 μM) but it did not induce oxidative DNA damage.
AB - © 2016 Elsevier Ltd Garlic (Allium sativum) and onion (Allium cepa) are being used in the food industry as flavoring but also for their antimicrobial activities. These activities are mainly derived from the organosulfur compounds (OSCs). Propyl propane thiosulfinate (PTS) is an OSC with potential use in the active packaging, but its safety should be guaranteed before being commercialized. The aim of this work was to investigate for the first time the cytotoxicity of PTS as well as its in vitro mutagenic/genotoxic potential using the following battery of genotoxicity tests:(1)the bacterial reverse-mutation assay in S. typhimurium (Ames test, OECD 471, 1997); (2) the micronucleus test (MN, OECD 487, 2016); (3) the mouse lymphoma thymidine-kinase assay (MLA, OECD 476, 2015), and (4) the comet assay (standard and modified with restriction enzymes). The results revealed that PTS was not mutagenic neither in the Ames test nor in MLA. However, genotoxic effects were recorded in the MN test on mammalian cells (L5178YTk+/−cells) after PTS exposure at the highest concentration tested (17.25 μM) without S9, and also its metabolites (+S9, from 20 μM). Moreover, in the comet assay, PTS induced DNA breaks damage in Caco-2 cells at the highest concentration tested (280 μM) but it did not induce oxidative DNA damage.
KW - Ames test
KW - Comet assay
KW - Micronucleus test
KW - Mouse lymphoma TK assay
KW - Propyl propane thiosulfinate (PTS)
U2 - https://doi.org/10.1016/j.fct.2016.12.007
DO - https://doi.org/10.1016/j.fct.2016.12.007
M3 - Article
SN - 0278-6915
VL - 99
SP - 231
EP - 240
JO - Food and Chemical Toxicology
JF - Food and Chemical Toxicology
ER -