The Pichia pastoris expression system is widely used for the production of recombinant proteins. A simple and efficient experimental set-up allowing on-line monitoring of the methanol concentration during the fermentation of P. pastorisbased on the detection of the methanol vapor concentration in the exhaust air from fermenter by a tin dioxide (SnO2) semiconductor sensor is described. An experimental procedure to allow precise calibration of the system and to reduce methanol sensor's interferences (>95% reduction) are also presented and discussed. Accuracy and measurement error were estimated about 0.05 g · l-1 and 6%, respectively. The efficient monitoring of methanol will help to advanced control of recombinant protein production and process optimization. © 2004 Kluwer Academic Publishers.
|Publication status||Published - 1 Sep 2004|
- Fermentation monitoring
- On-line methanol
- Pichia pastoris
Ramon, R., Feliu, J. X., Cos, O., Montesinos, J. L., Berthet, F. X., & Valero, F. (2004). Improving the monitoring of methanol concentration during high cell density fermentation of Pichia pastoris. Biotechnology Letters, 26, 1447-1452. https://doi.org/10.1023/B:BILE.0000045649.60508.c5