Improving the monitoring of methanol concentration during high cell density fermentation of Pichia pastoris

R. Ramon, J. X. Feliu, O. Cos, J. L. Montesinos, F. X. Berthet, F. Valero

Research output: Contribution to journalArticleResearchpeer-review

19 Citations (Scopus)

Abstract

The Pichia pastoris expression system is widely used for the production of recombinant proteins. A simple and efficient experimental set-up allowing on-line monitoring of the methanol concentration during the fermentation of P. pastorisbased on the detection of the methanol vapor concentration in the exhaust air from fermenter by a tin dioxide (SnO2) semiconductor sensor is described. An experimental procedure to allow precise calibration of the system and to reduce methanol sensor's interferences (>95% reduction) are also presented and discussed. Accuracy and measurement error were estimated about 0.05 g · l-1 and 6%, respectively. The efficient monitoring of methanol will help to advanced control of recombinant protein production and process optimization. © 2004 Kluwer Academic Publishers.
Original languageEnglish
Pages (from-to)1447-1452
JournalBiotechnology Letters
Volume26
DOIs
Publication statusPublished - 1 Sep 2004

Keywords

  • Fermentation monitoring
  • On-line methanol
  • Pichia pastoris

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