Abstract
The Pichia pastoris expression system is widely used for the production of recombinant proteins. A simple and efficient experimental set-up allowing on-line monitoring of the methanol concentration during the fermentation of P. pastorisbased on the detection of the methanol vapor concentration in the exhaust air from fermenter by a tin dioxide (SnO2) semiconductor sensor is described. An experimental procedure to allow precise calibration of the system and to reduce methanol sensor's interferences (>95% reduction) are also presented and discussed. Accuracy and measurement error were estimated about 0.05 g · l-1 and 6%, respectively. The efficient monitoring of methanol will help to advanced control of recombinant protein production and process optimization. © 2004 Kluwer Academic Publishers.
Original language | English |
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Pages (from-to) | 1447-1452 |
Journal | Biotechnology Letters |
Volume | 26 |
DOIs | |
Publication status | Published - 1 Sep 2004 |
Keywords
- Fermentation monitoring
- On-line methanol
- Pichia pastoris