Improving sensitivity of gold nanoparticle-based lateral flow assays by using wax-printed pillars as delay barriers of microfluidics

Lourdes Rivas, Mariana Medina-Sánchez, Alfredo De La Escosura-Muñiz, Arben Merkoçi

    Research output: Contribution to journalArticleResearchpeer-review

    89 Citations (Scopus)

    Abstract

    © the Partner Organisations 2014. Although lateral flow assays (LFAs) are currently being used in some point-of-care applications (POC), they cannot still be extended to a broader range of analytes for which higher sensitivities and lower detection limits are required. To overcome such drawbacks, we propose here a simple and facile alternative based on the use of delay hydrophobic barriers fabricated by wax printing so as to improve LFA sensitivity. Several wax pillar patterns were printed onto the nitrocellulose membrane in order to produce delays as well as pseudoturbulence in the microcapillary flow. The effect of the proposed wax pillar-modified devices was also mathematically simulated, corroborating the experimental results obtained for the different patterns tested afterwards for detection of HIgG as model protein in a gold nanoparticle-based LFA. The effect of the introduction of such wax-printed pillars was a sensitivity improvement of almost 3-fold compared to the sensitivity of a conventional free-barrier LFA.
    Original languageEnglish
    Pages (from-to)4406-4414
    JournalLab on a Chip
    Volume14
    Issue number22
    DOIs
    Publication statusPublished - 21 Nov 2014

    Fingerprint Dive into the research topics of 'Improving sensitivity of gold nanoparticle-based lateral flow assays by using wax-printed pillars as delay barriers of microfluidics'. Together they form a unique fingerprint.

  • Cite this

    Rivas, L., Medina-Sánchez, M., De La Escosura-Muñiz, A., & Merkoçi, A. (2014). Improving sensitivity of gold nanoparticle-based lateral flow assays by using wax-printed pillars as delay barriers of microfluidics. Lab on a Chip, 14(22), 4406-4414. https://doi.org/10.1039/c4lc00972j