Improving biogas production from microalgae by enzymatic pretreatment

Fabiana Passos, Andrea Hom-Diaz, Paqui Blanquez, Teresa Vicent, Ivet Ferrer

Research output: Contribution to journalArticleResearchpeer-review

49 Citations (Scopus)


© 2015 Elsevier Ltd. In this study, enzymatic pretreatment of microalgal biomass was investigated under different conditions and evaluated using biochemical methane potential (BMP) tests. Cellulase, glucohydrolase and an enzyme mix composed of cellulase, glucohydrolase and xylanase were selected based on the microalgae cell wall composition (cellulose, hemicellulose, pectin and glycoprotein). All of them increased organic matter solubilisation, obtaining high values already after 6 h of pretreatment with an enzyme dose of 1% for cellulase and the enzyme mix. BMP tests with pretreated microalgae showed a methane yield increase of 8 and 15% for cellulase and the enzyme mix, respectively. Prospective research should evaluate enzymatic pretreatments in continuous anaerobic reactors so as to estimate the energy balance and economic cost of the process.
Original languageEnglish
Pages (from-to)347-351
JournalBioresource Technology
Publication statusPublished - 1 Jan 2016


  • Algae
  • Enzyme
  • Hydrolysis
  • Methane
  • Solubilisation


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