Improved non-radioactive Northern blot protocol for detecting low abundance mRNAs from mammalian tissues

Montserrat Solanas; Raquel Moral; Eduard Escrich

doi:10.1023/A:1005693928291

Improved non-radioactive Northern blot protocol for detecting low abundance mRNAs from mammalian tissues

Montserrat Solanas, Raquel Moral, Eduard Escrich

Department of Cellular Biology, Physiology and Immunology

Research output: Contribution to journal › Article › Research › peer-review

9 Citations (Scopus)

Abstract

A rapid and sensitive non-radioactive Northern blot protocol is described which has been optimised in several critical steps. This is based on a formaldehyde-denaturing agarose gel electrophoresis, an alkaline transfer, hybridisation with digoxigenin-DNA probes and detection with a chemiluminescent substrate. This method allows even low abundance mRNAs to be detected in total RNA samples from mammalian tissues.

Original language	English
Pages (from-to)	263-266
Journal	Biotechnology Letters
Volume	23
DOIs	https://doi.org/10.1023/A:1005693928291
Publication status	Published - 28 Feb 2001

Keywords

Chemiluminescence
Digoxigenin
Mammalian tissues
Non-radioactive
Northern blot

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10.1023/A:1005693928291

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title = "Improved non-radioactive Northern blot protocol for detecting low abundance mRNAs from mammalian tissues",

abstract = "A rapid and sensitive non-radioactive Northern blot protocol is described which has been optimised in several critical steps. This is based on a formaldehyde-denaturing agarose gel electrophoresis, an alkaline transfer, hybridisation with digoxigenin-DNA probes and detection with a chemiluminescent substrate. This method allows even low abundance mRNAs to be detected in total RNA samples from mammalian tissues.",

keywords = "Chemiluminescence, Digoxigenin, Mammalian tissues, Non-radioactive, Northern blot",

author = "Montserrat Solanas and Raquel Moral and Eduard Escrich",

year = "2001",

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doi = "10.1023/A:1005693928291",

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TY - JOUR

T1 - Improved non-radioactive Northern blot protocol for detecting low abundance mRNAs from mammalian tissues

AU - Solanas, Montserrat

AU - Moral, Raquel

AU - Escrich, Eduard

PY - 2001/2/28

Y1 - 2001/2/28

N2 - A rapid and sensitive non-radioactive Northern blot protocol is described which has been optimised in several critical steps. This is based on a formaldehyde-denaturing agarose gel electrophoresis, an alkaline transfer, hybridisation with digoxigenin-DNA probes and detection with a chemiluminescent substrate. This method allows even low abundance mRNAs to be detected in total RNA samples from mammalian tissues.

AB - A rapid and sensitive non-radioactive Northern blot protocol is described which has been optimised in several critical steps. This is based on a formaldehyde-denaturing agarose gel electrophoresis, an alkaline transfer, hybridisation with digoxigenin-DNA probes and detection with a chemiluminescent substrate. This method allows even low abundance mRNAs to be detected in total RNA samples from mammalian tissues.

KW - Chemiluminescence

KW - Digoxigenin

KW - Mammalian tissues

KW - Non-radioactive

KW - Northern blot

U2 - 10.1023/A:1005693928291

DO - 10.1023/A:1005693928291

M3 - Article

SN - 0141-5492

VL - 23

SP - 263

EP - 266

JO - Biotechnology Letters

JF - Biotechnology Letters

ER -

Improved non-radioactive Northern blot protocol for detecting low abundance mRNAs from mammalian tissues

Abstract

Keywords

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