Improved non-radioactive Northern blot protocol for detecting low abundance mRNAs from mammalian tissues

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Abstract

A rapid and sensitive non-radioactive Northern blot protocol is described which has been optimised in several critical steps. This is based on a formaldehyde-denaturing agarose gel electrophoresis, an alkaline transfer, hybridisation with digoxigenin-DNA probes and detection with a chemiluminescent substrate. This method allows even low abundance mRNAs to be detected in total RNA samples from mammalian tissues.
Original languageEnglish
Pages (from-to)263-266
JournalBiotechnology Letters
Volume23
DOIs
Publication statusPublished - 28 Feb 2001

Keywords

  • Chemiluminescence
  • Digoxigenin
  • Mammalian tissues
  • Non-radioactive
  • Northern blot

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