Importance of the galE gene on the virulence of Pasteurella multocida

Antonio R. Fernández De Henestrosa; Ignacio Badiola; Montserrat Saco; Ana M. Perez De Rozas; Susana Campoy; Jordi Barbé

doi:10.1016/S0378-1097(97)00347-9

Importance of the galE gene on the virulence of Pasteurella multocida

Antonio R. Fernández De Henestrosa, Ignacio Badiola, Montserrat Saco, Ana M. Perez De Rozas, Susana Campoy, Jordi Barbé

Department of Genetics and Microbiology

Research output: Contribution to journal › Article › Research › peer-review

27 Citations (Scopus)

Abstract

The galE gene of Pasteurella multocida has been isolated by complementing galE-defective mutants of Salmonella Salmonella typhimurium with a plasmid library of this organism. The complete nucleotide sequence of the P. multocida galE gene consists of 1017 nucleotides, encoding a predicted polypeptide of 339 amino acids. The deduced amino acid sequence displayed the highest identity (85%) to the GalE protein of Haemophilus influenzae. However, the gene organization surrounding the galE locus was different from that of H. influenzae. A galE-defective mutant of P. multocida was obtained by replacement of the active galE gene by a copy inactivated in vitro. The resulting galE mutant was highly attenuated as seen in a biological test carried out in a mouse model.

Original language	English
Pages (from-to)	311-316
Journal	FEMS Microbiology Letters
Volume	154
Issue number	2
DOIs	https://doi.org/10.1016/S0378-1097(97)00347-9
Publication status	Published - 15 Sept 1997

Keywords

Attenuated mutant
galEgene
Pasteurella multocida
Virulence gene

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title = "Importance of the galE gene on the virulence of Pasteurella multocida",

abstract = "The galE gene of Pasteurella multocida has been isolated by complementing galE-defective mutants of Salmonella Salmonella typhimurium with a plasmid library of this organism. The complete nucleotide sequence of the P. multocida galE gene consists of 1017 nucleotides, encoding a predicted polypeptide of 339 amino acids. The deduced amino acid sequence displayed the highest identity (85%) to the GalE protein of Haemophilus influenzae. However, the gene organization surrounding the galE locus was different from that of H. influenzae. A galE-defective mutant of P. multocida was obtained by replacement of the active galE gene by a copy inactivated in vitro. The resulting galE mutant was highly attenuated as seen in a biological test carried out in a mouse model.",

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T1 - Importance of the galE gene on the virulence of Pasteurella multocida

AU - Fernández De Henestrosa, Antonio R.

AU - Badiola, Ignacio

AU - Saco, Montserrat

AU - Perez De Rozas, Ana M.

AU - Campoy, Susana

AU - Barbé, Jordi

PY - 1997/9/15

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N2 - The galE gene of Pasteurella multocida has been isolated by complementing galE-defective mutants of Salmonella Salmonella typhimurium with a plasmid library of this organism. The complete nucleotide sequence of the P. multocida galE gene consists of 1017 nucleotides, encoding a predicted polypeptide of 339 amino acids. The deduced amino acid sequence displayed the highest identity (85%) to the GalE protein of Haemophilus influenzae. However, the gene organization surrounding the galE locus was different from that of H. influenzae. A galE-defective mutant of P. multocida was obtained by replacement of the active galE gene by a copy inactivated in vitro. The resulting galE mutant was highly attenuated as seen in a biological test carried out in a mouse model.

AB - The galE gene of Pasteurella multocida has been isolated by complementing galE-defective mutants of Salmonella Salmonella typhimurium with a plasmid library of this organism. The complete nucleotide sequence of the P. multocida galE gene consists of 1017 nucleotides, encoding a predicted polypeptide of 339 amino acids. The deduced amino acid sequence displayed the highest identity (85%) to the GalE protein of Haemophilus influenzae. However, the gene organization surrounding the galE locus was different from that of H. influenzae. A galE-defective mutant of P. multocida was obtained by replacement of the active galE gene by a copy inactivated in vitro. The resulting galE mutant was highly attenuated as seen in a biological test carried out in a mouse model.

KW - Attenuated mutant

KW - galEgene

KW - Pasteurella multocida

KW - Virulence gene

U2 - 10.1016/S0378-1097(97)00347-9

DO - 10.1016/S0378-1097(97)00347-9

M3 - Article

VL - 154

SP - 311

EP - 316

IS - 2

ER -

Importance of the galE gene on the virulence of Pasteurella multocida

Abstract

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