Importance of the galE gene on the virulence of Pasteurella multocida

Antonio R. Fernández De Henestrosa, Ignacio Badiola, Montserrat Saco, Ana M. Perez De Rozas, Susana Campoy, Jordi Barbé

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26 Citations (Scopus)

Abstract

The galE gene of Pasteurella multocida has been isolated by complementing galE-defective mutants of Salmonella Salmonella typhimurium with a plasmid library of this organism. The complete nucleotide sequence of the P. multocida galE gene consists of 1017 nucleotides, encoding a predicted polypeptide of 339 amino acids. The deduced amino acid sequence displayed the highest identity (85%) to the GalE protein of Haemophilus influenzae. However, the gene organization surrounding the galE locus was different from that of H. influenzae. A galE-defective mutant of P. multocida was obtained by replacement of the active galE gene by a copy inactivated in vitro. The resulting galE mutant was highly attenuated as seen in a biological test carried out in a mouse model.
Original languageEnglish
Pages (from-to)311-316
JournalFEMS Microbiology Letters
Volume154
Issue number2
DOIs
Publication statusPublished - 15 Sep 1997

Keywords

  • Attenuated mutant
  • galEgene
  • Pasteurella multocida
  • Virulence gene

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