Impedimetric genosensors for the detection of DNA hybridization

A. Bonanni; M. J. Esplandiu; M. I. Pividori; S. Alegret; M. Del Valle

doi:10.1007/s00216-006-0558-3

Impedimetric genosensors for the detection of DNA hybridization

A. Bonanni, M. J. Esplandiu, M. I. Pividori, S. Alegret, M. Del Valle

Department of Chemistry

Research output: Contribution to journal › Article › Research › peer-review

57 Citations (Scopus)

Abstract

Impedance spectroscopy is proposed as the transduction principle for detecting the hybridization of DNA complementary strands. In our experiments, different DNA oligonucleotides were used as model gene substances. The gene probe is first immobilized on a graphite-epoxy composite working electrode based genosensor. Detection principle is based on changes of impedance spectra of a redox marker, the ferro/ferricyanide couple, after hybridization with target DNA. Resistance offered to the electrochemical reaction serves as the working signal, allowing for an unlabelled gene assay. [Figure not available: see fulltext.].

Original language	English
Pages (from-to)	1195-1201
Journal	Analytical and Bioanalytical Chemistry
Volume	385
Issue number	7
DOIs	https://doi.org/10.1007/s00216-006-0558-3
Publication status	Published - 1 Aug 2006

Keywords

Capacitance
DNA biosensor
Impedance
Unlabelled DNA

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title = "Impedimetric genosensors for the detection of DNA hybridization",

abstract = "Impedance spectroscopy is proposed as the transduction principle for detecting the hybridization of DNA complementary strands. In our experiments, different DNA oligonucleotides were used as model gene substances. The gene probe is first immobilized on a graphite-epoxy composite working electrode based genosensor. Detection principle is based on changes of impedance spectra of a redox marker, the ferro/ferricyanide couple, after hybridization with target DNA. Resistance offered to the electrochemical reaction serves as the working signal, allowing for an unlabelled gene assay. [Figure not available: see fulltext.].",

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T1 - Impedimetric genosensors for the detection of DNA hybridization

AU - Bonanni, A.

AU - Esplandiu, M. J.

AU - Pividori, M. I.

AU - Alegret, S.

AU - Del Valle, M.

PY - 2006/8/1

Y1 - 2006/8/1

N2 - Impedance spectroscopy is proposed as the transduction principle for detecting the hybridization of DNA complementary strands. In our experiments, different DNA oligonucleotides were used as model gene substances. The gene probe is first immobilized on a graphite-epoxy composite working electrode based genosensor. Detection principle is based on changes of impedance spectra of a redox marker, the ferro/ferricyanide couple, after hybridization with target DNA. Resistance offered to the electrochemical reaction serves as the working signal, allowing for an unlabelled gene assay. [Figure not available: see fulltext.].

AB - Impedance spectroscopy is proposed as the transduction principle for detecting the hybridization of DNA complementary strands. In our experiments, different DNA oligonucleotides were used as model gene substances. The gene probe is first immobilized on a graphite-epoxy composite working electrode based genosensor. Detection principle is based on changes of impedance spectra of a redox marker, the ferro/ferricyanide couple, after hybridization with target DNA. Resistance offered to the electrochemical reaction serves as the working signal, allowing for an unlabelled gene assay. [Figure not available: see fulltext.].

KW - Capacitance

KW - DNA biosensor

KW - Impedance

KW - Unlabelled DNA

U2 - 10.1007/s00216-006-0558-3

DO - 10.1007/s00216-006-0558-3

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