Impedimetric genosensors for the detection of DNA hybridization

A. Bonanni, M. J. Esplandiu, M. I. Pividori, S. Alegret, M. Del Valle

Research output: Contribution to journalArticleResearchpeer-review

57 Citations (Scopus)

Abstract

Impedance spectroscopy is proposed as the transduction principle for detecting the hybridization of DNA complementary strands. In our experiments, different DNA oligonucleotides were used as model gene substances. The gene probe is first immobilized on a graphite-epoxy composite working electrode based genosensor. Detection principle is based on changes of impedance spectra of a redox marker, the ferro/ferricyanide couple, after hybridization with target DNA. Resistance offered to the electrochemical reaction serves as the working signal, allowing for an unlabelled gene assay. [Figure not available: see fulltext.].
Original languageEnglish
Pages (from-to)1195-1201
JournalAnalytical and Bioanalytical Chemistry
Volume385
Issue number7
DOIs
Publication statusPublished - 1 Aug 2006

Keywords

  • Capacitance
  • DNA biosensor
  • Impedance
  • Unlabelled DNA

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