TY - JOUR
T1 - Impedimetric approach for quantifying low bacteria concentrations based on the changes produced in the electrode-solution interface during the pre-attachment stage
AU - Muñoz-Berbel, X.
AU - Vigués, N.
AU - Jenkins, A. T.A.
AU - Mas, J.
AU - Muñoz, F. J.
PY - 2008/5/15
Y1 - 2008/5/15
N2 - This paper describes an approach for quantifying low concentrations of bacteria, particularly Escherichia coli, based on the measurement of the initial attachment of bacteria to platinum surfaces, using impedance spectroscopy. The value of the interface capacitance in the pre-attachment stage (before 1 min of attachment) showed correlation with suspended concentration of bacteria from 101 to 107 CFU mL-1 (colony forming units per mL). This method was found to be sensitive to the attachment time, to the applied potential and to the size of the counter electrode. The sensor lifetime was also evaluated. © 2008 Elsevier B.V. All rights reserved.
AB - This paper describes an approach for quantifying low concentrations of bacteria, particularly Escherichia coli, based on the measurement of the initial attachment of bacteria to platinum surfaces, using impedance spectroscopy. The value of the interface capacitance in the pre-attachment stage (before 1 min of attachment) showed correlation with suspended concentration of bacteria from 101 to 107 CFU mL-1 (colony forming units per mL). This method was found to be sensitive to the attachment time, to the applied potential and to the size of the counter electrode. The sensor lifetime was also evaluated. © 2008 Elsevier B.V. All rights reserved.
KW - Bacterial attachment
KW - Biomass quantification
KW - Impedance spectroscopy
KW - Platinum electrodes
KW - Surface plasmon resonance
U2 - 10.1016/j.bios.2008.01.007
DO - 10.1016/j.bios.2008.01.007
M3 - Article
SN - 0956-5663
VL - 23
SP - 1540
EP - 1546
JO - Biosensors and Bioelectronics
JF - Biosensors and Bioelectronics
ER -