Impact of CSF storage volume on the analysis of Alzheimer's disease biomarkers on an automated platform

Constance Delaby; Laia Muñoz; Soraya Torres; Alícia Nadal; Nathalie Le Bastard; Sylvain Lehmann; Alberto Lleó; Daniel Alcolea

doi:10.1016/j.cca.2018.12.021

Impact of CSF storage volume on the analysis of Alzheimer's disease biomarkers on an automated platform

Constance Delaby, Laia Muñoz, Soraya Torres, Alícia Nadal, Nathalie Le Bastard, Sylvain Lehmann, Alberto Lleó, Daniel Alcolea

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Abstract

© 2018 Elsevier B.V. Objectives: To assess the potential influence of the ratio between the storage tube surface area and the volume of cerebrospinal fluid (CSF) (surface/volume) on the quantifications of four Alzheimer's disease (AD) biomarkers on the Lumipulse G600II automated platform. Methods: CSF samples of 10 consecutive patients were stored in 2 ml polypropylene tubes containing four different CSF volumes: 1.5 ml, 1 ml, 0.5 ml and 0.25 ml. Concentration of CSF Aβ1-42, Aβ1–40, t-Tau and p-Tau were measured in all aliquots using the LUMIPULSE G600II automated platform from Fujirebio. Results: Levels of CSF Aβ1-42 and Aβ1-40 were lower in samples stored with lower volumes (higher surface/volume ratios). This decrease was partly compensated by using the ratio Aβ1-42/Aβ1-40. Quantification of t-Tau and p-Tau were not influenced by this pre-analytical condition. Conclusion: The surface/volume ratio can potentially influence the results of amyloid AD biomarkers. It appears essential to take into account the surface/volume ratio of the storage tubes when quantifying CSF biomarkers in clinical routine.

Original language	English
Pages (from-to)	98-101
Journal	Clinica Chimica Acta
Volume	490
DOIs	https://doi.org/10.1016/j.cca.2018.12.021
Publication status	Published - 1 Mar 2019

Keywords

Alzheimer's disease
Amyloid
Biomarkers
Cerebrospinal fluid
Lumipulse
Tau

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10.1016/j.cca.2018.12.021

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title = "Impact of CSF storage volume on the analysis of Alzheimer's disease biomarkers on an automated platform",

abstract = "{\textcopyright} 2018 Elsevier B.V. Objectives: To assess the potential influence of the ratio between the storage tube surface area and the volume of cerebrospinal fluid (CSF) (surface/volume) on the quantifications of four Alzheimer's disease (AD) biomarkers on the Lumipulse G600II automated platform. Methods: CSF samples of 10 consecutive patients were stored in 2 ml polypropylene tubes containing four different CSF volumes: 1.5 ml, 1 ml, 0.5 ml and 0.25 ml. Concentration of CSF Aβ1-42, Aβ1–40, t-Tau and p-Tau were measured in all aliquots using the LUMIPULSE G600II automated platform from Fujirebio. Results: Levels of CSF Aβ1-42 and Aβ1-40 were lower in samples stored with lower volumes (higher surface/volume ratios). This decrease was partly compensated by using the ratio Aβ1-42/Aβ1-40. Quantification of t-Tau and p-Tau were not influenced by this pre-analytical condition. Conclusion: The surface/volume ratio can potentially influence the results of amyloid AD biomarkers. It appears essential to take into account the surface/volume ratio of the storage tubes when quantifying CSF biomarkers in clinical routine.",

keywords = "Alzheimer's disease, Amyloid, Biomarkers, Cerebrospinal fluid, Lumipulse, Tau",

author = "Constance Delaby and Laia Mu{\~n}oz and Soraya Torres and Al{\'i}cia Nadal and {Le Bastard}, Nathalie and Sylvain Lehmann and Alberto Lle{\'o} and Daniel Alcolea",

year = "2019",

month = mar,

day = "1",

doi = "10.1016/j.cca.2018.12.021",

language = "English",

volume = "490",

pages = "98--101",

journal = "Clinica Chimica Acta",

issn = "0009-8981",

publisher = "Elsevier",

}

TY - JOUR

T1 - Impact of CSF storage volume on the analysis of Alzheimer's disease biomarkers on an automated platform

AU - Delaby, Constance

AU - Muñoz, Laia

AU - Torres, Soraya

AU - Nadal, Alícia

AU - Le Bastard, Nathalie

AU - Lehmann, Sylvain

AU - Lleó, Alberto

AU - Alcolea, Daniel

PY - 2019/3/1

Y1 - 2019/3/1

N2 - © 2018 Elsevier B.V. Objectives: To assess the potential influence of the ratio between the storage tube surface area and the volume of cerebrospinal fluid (CSF) (surface/volume) on the quantifications of four Alzheimer's disease (AD) biomarkers on the Lumipulse G600II automated platform. Methods: CSF samples of 10 consecutive patients were stored in 2 ml polypropylene tubes containing four different CSF volumes: 1.5 ml, 1 ml, 0.5 ml and 0.25 ml. Concentration of CSF Aβ1-42, Aβ1–40, t-Tau and p-Tau were measured in all aliquots using the LUMIPULSE G600II automated platform from Fujirebio. Results: Levels of CSF Aβ1-42 and Aβ1-40 were lower in samples stored with lower volumes (higher surface/volume ratios). This decrease was partly compensated by using the ratio Aβ1-42/Aβ1-40. Quantification of t-Tau and p-Tau were not influenced by this pre-analytical condition. Conclusion: The surface/volume ratio can potentially influence the results of amyloid AD biomarkers. It appears essential to take into account the surface/volume ratio of the storage tubes when quantifying CSF biomarkers in clinical routine.

AB - © 2018 Elsevier B.V. Objectives: To assess the potential influence of the ratio between the storage tube surface area and the volume of cerebrospinal fluid (CSF) (surface/volume) on the quantifications of four Alzheimer's disease (AD) biomarkers on the Lumipulse G600II automated platform. Methods: CSF samples of 10 consecutive patients were stored in 2 ml polypropylene tubes containing four different CSF volumes: 1.5 ml, 1 ml, 0.5 ml and 0.25 ml. Concentration of CSF Aβ1-42, Aβ1–40, t-Tau and p-Tau were measured in all aliquots using the LUMIPULSE G600II automated platform from Fujirebio. Results: Levels of CSF Aβ1-42 and Aβ1-40 were lower in samples stored with lower volumes (higher surface/volume ratios). This decrease was partly compensated by using the ratio Aβ1-42/Aβ1-40. Quantification of t-Tau and p-Tau were not influenced by this pre-analytical condition. Conclusion: The surface/volume ratio can potentially influence the results of amyloid AD biomarkers. It appears essential to take into account the surface/volume ratio of the storage tubes when quantifying CSF biomarkers in clinical routine.

KW - Alzheimer's disease

KW - Amyloid

KW - Biomarkers

KW - Cerebrospinal fluid

KW - Lumipulse

KW - Tau

U2 - 10.1016/j.cca.2018.12.021

DO - 10.1016/j.cca.2018.12.021

M3 - Article

C2 - 30579960

VL - 490

SP - 98

EP - 101

JO - Clinica Chimica Acta

JF - Clinica Chimica Acta

SN - 0009-8981

ER -

Impact of CSF storage volume on the analysis of Alzheimer's disease biomarkers on an automated platform

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Keywords

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