Immunomagnetic Separation of Salmonella with Tailored Magnetic Micro- and Nanocarriers.

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Abstract

One of the main drawbacks in current methods for bacterium detection is their quantification at very low concentration level in complex specimens. Novel developments that are needed involve solid-phase preconcentration procedures which can be easily integrated with emerging technologies. Here, we describe the immunomagnetic separation (IMS) of Salmonella using magnetic carriers. Nano (300 nm) and micro (2.8 μm) sized magnetic particles are modified with anti-Salmonella antibody to preconcentrate the bacteria from the samples throughout an immunological reaction. The immunomagnetic separation can be easily coupled with downstream characterization and quantification methods, including classical culturing, molecular biology techniques such as PCR, immunoassays, confocal and scanning electron microscopy, and emerging technologies and rapid detection methods including biosensors, lateral flow, and microfluidic devices.

Original languageEnglish
Pages (from-to)51-65
Number of pages15
JournalMethods in molecular biology (Clifton, N.J.)
Volume2182
DOIs
Publication statusPublished - 8 Sep 2020

Keywords

  • Antibodies
  • Confocal microscopy
  • ELISA
  • Immunomagnetic separation
  • Magnetic nanoparticles
  • Salmonella enterica
  • Scanning electron microscopy
  • Solid-phase preconcentration
  • Limit of Detection
  • Immunoassay/methods
  • Lab-On-A-Chip Devices
  • Immunomagnetic Separation/methods
  • Magnetics/methods
  • Polymerase Chain Reaction/methods
  • Magnetic Phenomena
  • Salmonella/genetics

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