TY - JOUR
T1 - Immunomagnetic separation of Salmonella with tailored magnetic micro and nanocarriers. A comparative study
AU - Brandão, Delfina
AU - Liébana, Susana
AU - Campoy, Susana
AU - Alegret, Salvador
AU - Pividori, María Isabel
PY - 2015/6/9
Y1 - 2015/6/9
N2 - © 2015 Elsevier B.V. Abstract This paper addresses a comparative study of immunomagnetic separation of Salmonella using micro and nano-sized magnetic carriers. In this approach, nano (300 nm) and micro (2.8 μm) sized magnetic particles were modified with anti-Salmonella antibody to pre-concentrate the bacteria from the samples throughout an immunological reaction. The performance of the immunomagnetic separation on the different magnetic carriers was evaluated using classical culturing, confocal and scanning electron microscopy to study the binding pattern, as well as a magneto-actuated immunosensor with electrochemical read-out for the rapid detection of the bacteria in spiked milk samples. In this approach, a second polyclonal antibody labeled with peroxidase as electrochemical reporter was used. The magneto-actuated electrochemical immunosensor was able to clearly distinguish between food pathogenic bacteria such as Salmonella enterica and Escherichia coli, showing a limit of detection (LOD) as low as 538 CFU mL-1 and 291 CFU mL-1 for magnetic micro and nanocarriers, respectively, in whole milk, although magnetic nanoparticles showed a noticeable higher matrix effect and higher agglomeration effect. These LODs were achieved in a total assay time of 1 h without any previous culturing pre-enrichment step. If the samples were pre-enriched for 8 h, the magneto immunosensor based on the magnetic nanoparticles was able to detect as low as 1 CFU in 25 mL of milk (0.04 CFU mL-1).
AB - © 2015 Elsevier B.V. Abstract This paper addresses a comparative study of immunomagnetic separation of Salmonella using micro and nano-sized magnetic carriers. In this approach, nano (300 nm) and micro (2.8 μm) sized magnetic particles were modified with anti-Salmonella antibody to pre-concentrate the bacteria from the samples throughout an immunological reaction. The performance of the immunomagnetic separation on the different magnetic carriers was evaluated using classical culturing, confocal and scanning electron microscopy to study the binding pattern, as well as a magneto-actuated immunosensor with electrochemical read-out for the rapid detection of the bacteria in spiked milk samples. In this approach, a second polyclonal antibody labeled with peroxidase as electrochemical reporter was used. The magneto-actuated electrochemical immunosensor was able to clearly distinguish between food pathogenic bacteria such as Salmonella enterica and Escherichia coli, showing a limit of detection (LOD) as low as 538 CFU mL-1 and 291 CFU mL-1 for magnetic micro and nanocarriers, respectively, in whole milk, although magnetic nanoparticles showed a noticeable higher matrix effect and higher agglomeration effect. These LODs were achieved in a total assay time of 1 h without any previous culturing pre-enrichment step. If the samples were pre-enriched for 8 h, the magneto immunosensor based on the magnetic nanoparticles was able to detect as low as 1 CFU in 25 mL of milk (0.04 CFU mL-1).
KW - Electrochemical immunosensor
KW - Immunomagnetic separation
KW - Magnetic nanoparticles
KW - Salmonella enterica
U2 - 10.1016/j.talanta.2015.05.035
DO - 10.1016/j.talanta.2015.05.035
M3 - Article
SN - 0039-9140
VL - 143
SP - 198
EP - 204
JO - Talanta
JF - Talanta
M1 - 15627
ER -