Immunohistochemical study of doublecortin and nucleostemin in canine brain

Marti Pumarola Batlle, Maria Dolores Perez Rodriguez, Ester Blasco Ortega, M. Dolores Fondevila Palau, Paola Marco Salazar, Eleonora de Nevi

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15 Citations (Scopus)


Finding a marker of neural stem cells remains a medical research priority. It was reported that the proteins doublecortin and nucleostemin were related with stem/progenitor cells in central nervous system. The aim of the present immunohistochemical study was to evaluate the expression of these proteins and their pattern of distribution in canine brain, including age-related changes, and in non-nervous tissues. We found that doublecortin had a more specific expression pattern, related with neurogenesis and neuronal migration, while nucleostemin was expressed in most cells of almost every tissue studied. The immunolabeling of both proteins decreased with age. We may conclude that nucleostemin is not a specific marker of stem/progenitor cells in the dog. Doublecortin, however, is not an exclusive marker of neural stem cells, but also of neuronal precursors.

Original languageEnglish
Pages (from-to)55-59
Number of pages5
JournalEuropean Journal of Histochemistry
Issue number1
Publication statusPublished - 1 Jan 2013


  • Aging
  • Dog brain
  • Doublecortin
  • Nucleostemin
  • Stem cells


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