TY - JOUR
T1 - Immunohistochemical study of doublecortin and nucleostemin in canine brain
AU - Pumarola Batlle, Marti
AU - Perez Rodriguez, Maria Dolores
AU - Blasco Ortega, Ester
AU - Fondevila Palau, M. Dolores
AU - Marco Salazar, Paola
AU - de Nevi, Eleonora
N1 - Copyright:
Copyright 2018 Elsevier B.V., All rights reserved.
PY - 2013/1/1
Y1 - 2013/1/1
N2 - Finding a marker of neural stem cells remains a medical research priority. It was reported that the proteins doublecortin and nucleostemin were related with stem/progenitor cells in central nervous system. The aim of the present immunohistochemical study was to evaluate the expression of these proteins and their pattern of distribution in canine brain, including age-related changes, and in non-nervous tissues. We found that doublecortin had a more specific expression pattern, related with neurogenesis and neuronal migration, while nucleostemin was expressed in most cells of almost every tissue studied. The immunolabeling of both proteins decreased with age. We may conclude that nucleostemin is not a specific marker of stem/progenitor cells in the dog. Doublecortin, however, is not an exclusive marker of neural stem cells, but also of neuronal precursors.
AB - Finding a marker of neural stem cells remains a medical research priority. It was reported that the proteins doublecortin and nucleostemin were related with stem/progenitor cells in central nervous system. The aim of the present immunohistochemical study was to evaluate the expression of these proteins and their pattern of distribution in canine brain, including age-related changes, and in non-nervous tissues. We found that doublecortin had a more specific expression pattern, related with neurogenesis and neuronal migration, while nucleostemin was expressed in most cells of almost every tissue studied. The immunolabeling of both proteins decreased with age. We may conclude that nucleostemin is not a specific marker of stem/progenitor cells in the dog. Doublecortin, however, is not an exclusive marker of neural stem cells, but also of neuronal precursors.
KW - Aging
KW - Dog brain
KW - Doublecortin
KW - Nucleostemin
KW - Stem cells
UR - http://www.scopus.com/inward/record.url?scp=84875289785&partnerID=8YFLogxK
U2 - 10.4081/ejh.2013.e9
DO - 10.4081/ejh.2013.e9
M3 - Article
C2 - 23549468
SN - 1121-760X
VL - 57
SP - 55
EP - 59
JO - European Journal of Histochemistry
JF - European Journal of Histochemistry
IS - 1
ER -