TY - JOUR
T1 - Immunohistochemical localization of the sigma1 receptor in Schwann cells of rat sciatic nerve
AU - Palacios, Gabriel
AU - Muro, Asunción
AU - Verdú, Enric
AU - Pumarola, Martí
AU - Vela, José Miguel
N1 - Copyright:
Copyright 2008 Elsevier B.V., All rights reserved.
PY - 2004/5/8
Y1 - 2004/5/8
N2 - The sigma-1 (σ1) receptors can bind different psychotropic drugs and have been implicated in schizophrenia, depression and dementia. The cloning of the σ1-receptor has allowed to obtain specific antibodies and, in a recent immunohistochemical study, we demonstrated that, in addition to neurons, the σ1-receptor is located in oligodendrocytes [Brain Res. 961 (2003) 92.]. In the present study using in vivo and in vitro techniques, we demonstrate the localization of the σ1-receptor in Schwann cells. Double immunofluorescence studies showed that σ1-receptor co-localized with S100 protein, a specific marker of Schwann cells, in both rat sciatic nerve Schwann cells and Schwann cells in cultures. The σ1-receptor immunoreactivity was seen in the cytoplasm and paranodal region formed by these cells, but not in myelin itself. The presence of σ1-receptor in oligodendrocytes and Schwann cells is discussed on the basis on recent findings involving this receptor in lipid metabolism, compartmentalization and transport to the plasma membrane, thus suggesting a role for σ1-receptor signaling in myelination. © 2004 Elsevier B.V. All rights reserved.
AB - The sigma-1 (σ1) receptors can bind different psychotropic drugs and have been implicated in schizophrenia, depression and dementia. The cloning of the σ1-receptor has allowed to obtain specific antibodies and, in a recent immunohistochemical study, we demonstrated that, in addition to neurons, the σ1-receptor is located in oligodendrocytes [Brain Res. 961 (2003) 92.]. In the present study using in vivo and in vitro techniques, we demonstrate the localization of the σ1-receptor in Schwann cells. Double immunofluorescence studies showed that σ1-receptor co-localized with S100 protein, a specific marker of Schwann cells, in both rat sciatic nerve Schwann cells and Schwann cells in cultures. The σ1-receptor immunoreactivity was seen in the cytoplasm and paranodal region formed by these cells, but not in myelin itself. The presence of σ1-receptor in oligodendrocytes and Schwann cells is discussed on the basis on recent findings involving this receptor in lipid metabolism, compartmentalization and transport to the plasma membrane, thus suggesting a role for σ1-receptor signaling in myelination. © 2004 Elsevier B.V. All rights reserved.
KW - Cellular and molecular biology
KW - Immunohistochemistry
KW - Myelination
KW - Neuroglia and myelin
KW - Rat sciatic nerve
KW - Schwann cell
KW - Sigma (σ)-receptor
UR - http://www.scopus.com/inward/record.url?scp=1942535098&partnerID=8YFLogxK
U2 - 10.1016/j.brainres.2004.02.013
DO - 10.1016/j.brainres.2004.02.013
M3 - Article
C2 - 15064136
VL - 1007
SP - 65
EP - 70
IS - 1-2
ER -