Immunization with DNA vaccines containing porcine reproductive and respiratory syndrome virus open reading frames 5, 6, and 7 may be related to the exacerbation of clinical disease after an experimental challenge

Ivan Díaz, Llilianne Ganges, Iván Galindo-Cardiel, Joan Tarradas, Belén Álvarez, Cristina Lorca-Oró, Joan Pujols, Mariona Gimeno, Laila Darwich, Mariano Domingo, Javier Domínguez, Enric Mateu

Research output: Contribution to journalArticleResearchpeer-review

9 Citations (Scopus)

Abstract

Pigs were immunized with DNA plasmids containing different open reading frames (ORFs) of a porcine reproductive and respiratory syndrome virus (PRRSV) genotype I strain. One group was injected with three inoculations of ORF7, a second group was immunized with three inoculations of plasmids containing ORF5 and ORF6, and a third group was kept as controls. Later, +21 days after the last inoculation, animals were challenged with the homologous strain. After the challenge, PRRSV-specific interferon (IFN)-γ-secreting cells and anti-PRRSV IgG antibodies developed faster in DNA vaccinated pigs (p<0.05). However, DNA-immunized pigs showed an exacerbation of the disease compared to the unvaccinated challenged pigs. The data suggest that previous immunization with DNA vaccines against glycoprotein 5 and/or matrix protein of PRRSV, as well as nucleoprotein but to a lesser degree, could result in an exacerbation of the clinical course in terms of fever upon challenge. © Mary Ann Liebert, Inc. 2013.
Original languageEnglish
Pages (from-to)93-101
JournalViral Immunology
Volume26
DOIs
Publication statusPublished - 1 Feb 2013

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