Immunization of mice with a Mycobacterium tuberculosis genomic expression library results in lower bacterial load in lungs after challenge with BCG

Yamilé López Hernández, Daniel Yero Corona, Sergio Sifontes Rodríguez, Juan Francisco Infante Bourzac, Marìa Elena Sarmiento, Nesty Olivares Arzuaga, Enrique Casado Maceo, Daiyana Díaz, Raùl Díaz, Armando Acosta Domínguez

Research output: Contribution to journalArticleResearchpeer-review

7 Citations (Scopus)

Abstract

Tuberculosis is a serious infectious disease in many developing countries. The lack of an effective vaccine for preventing this disease has stimulated the search for new vaccine candidates against Mycobacterium tuberculosis. In the present work, the construction of a genomic expression library of M. tuberculosis in a eukaryotic expression vector was carried out. Immunization of Balb/c mice with a plasmid DNA pool from this library (containing 8360 clones) induced a significant IgG antibody response. Immunized mice were challenged by intratracheal route with 10(5) cfu of non-pathogenic Mycobacterium bovis BCG and were sacrificed 21 days post-challenge. Mice immunized with the genomic expression library showed a significant reduction of viable bacteria in lungs and less pulmonary tissue damage. Granulomas were not observed and the lungs had a more discrete perivascular inflammatory cell infiltrate compared to control mice. Results suggest that the genomic expression library contains genes encoding proteins that are protective against M. tuberculosis infection.

Original languageAmerican English
Pages (from-to)247-54
Number of pages8
JournalTuberculosis
Volume86
Issue number3-4
DOIs
Publication statusPublished - 2 May 2006

Keywords

  • Animals
  • Antibodies, Bacterial/biosynthesis
  • Antigens, Bacterial/genetics
  • DNA, Bacterial/immunology
  • Genome, Bacterial
  • Genomic Library
  • Immunization
  • Immunoglobulin G/biosynthesis
  • Lung/microbiology
  • Mice
  • Mice, Inbred BALB C
  • Mycobacterium bovis/immunology
  • Tuberculosis/immunology
  • Tuberculosis Vaccines/immunology
  • Vaccines, DNA/immunology

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