Immune response of drosophila suzukii larvae to infection with the nematobacterial complex steinernema carpocapsae–xenorhabdus nematophila

Anna Garriga, Maristella Mastore, Ana Morton, Fernando Garcia Del Pino*, Maurizio Francesco Brivio

*Corresponding author for this work

Research output: Contribution to journalArticleResearchpeer-review

10 Citations (Scopus)

Abstract

Entomopathogenic nematodes have been proposed as biological agents for the control of Drosophila suzukii, an invasive pest of small-stone and soft-skinned fruits. Larvae of the fly are susceptible to Steinernema carpocapsae infection but the reaction of immune defenses of the host are unknown. To determine the immune response, larvae were infected with S. carpocapsae and Xenorhabdus nematophila to evaluate the effector mechanisms of both humoral and cellular processes. The symbiont bacteria presented an inhibitory effect on the phenoloxidase cascade with a low level of melanization. Besides, X. nematophila activated the synthesis of putative antimicrobial peptides on the hemolymph of infected larvae. However, those peptides presented a lower antimicrobial activity compared to hemolymph from larvae infected with non-symbiont bacteria. Xenorhabdus nematophila avoided also the phagocytosis response of hemocytes. During in vitro and in vivo assays, S. carpocapsae was not encapsulated by cells, unless the cuticle was damaged with a lipase-treatment. Hemocyte counts confirmed differentiation of lamellocytes in the early phase of infection despite the unrecognition of the nematodes. Both X. nematophila and S. carpocapsae avoided the cellular defenses of D. suzukii larvae and depressed the humoral response. These results confirmed the potential of entomopathogenic nematodes to control D. suzukii.

Original languageEnglish
Article number210
JournalInsects
Volume11
Issue number4
DOIs
Publication statusPublished - Apr 2020

Keywords

  • Cellular defenses
  • Drosophila suzukii
  • Entomopathogenic nematodes
  • Humoral defenses
  • Immunity
  • Steinernema carpocapsae
  • Xenorhabdus nematophila

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