Immobilized heterologous Rhizopus Oryzae lipase as an efficient catalyst in the acetylation of cortexolone

Paula G. Quintana, Marina Guillén, Marzia Marciello, Francisco Valero, Jose M. Palomo, Alicia Baldessari

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    Abstract

    The enzymatic preparation of a monoacetyl derivative of the corticosteroid cortexolone, through a transesterification reaction, is described. The heterologous Rhizopus oryzae lipase, immobilized on three different supports, proved to be an efficient catalyst in the acylation reaction using a complex substrate such as cortexolone. Immobilization of the enzyme on Lewatit 1600 resin at pH = 7 and 25 °C was the best condition for catalysis of the acetylation reaction. The influence of various reaction parameters, such as the nature of the acetylating agent, the solvent, the temperature, and the ratios of acetylating agent to substrate, and enzyme to substrate, was evaluated. Using the response surface methodology and a central composite rotatable design, the specific yield of acetylated cortexolone was optimized by means of the study of the effect of the enzyme (E)/substrate (S) and the acylating agent (A)/substrate ratios. The ratios of 5 (E/S) and 31.6 (A/S) were predicted as the optimal values to reach the maximum specific yield of the product (P): 1.59 mmol P/mmol A·g E. The mild reaction conditions and low environmental impact make the biocatalytic procedure a convenient way to prepare the reported derivative of this biologically active steroid. The regioselective acetylation of cortexolone was achieved by using an immobilized heterologous Rhizopus oryzae lipase. The mild reaction conditions and low environmental impact make the biocatalytic procedure a convenient way to prepare the monoacetyl derivative of this biologically active steroid. Copyright © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
    Original languageEnglish
    Pages (from-to)4306-4312
    JournalEuropean Journal of Organic Chemistry
    DOIs
    Publication statusPublished - 1 Aug 2012

    Keywords

    • Acylation
    • Biocatalysis
    • Enzymes
    • Immobilization
    • Steroids
    • Supported catalysts

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  • Cite this

    Quintana, P. G., Guillén, M., Marciello, M., Valero, F., Palomo, J. M., & Baldessari, A. (2012). Immobilized heterologous Rhizopus Oryzae lipase as an efficient catalyst in the acetylation of cortexolone. European Journal of Organic Chemistry, 4306-4312. https://doi.org/10.1002/ejoc.201200178