The HB autoantigen, a 10-kDa DNA-binding protein recognized by autoantibodies only when bound to DNA, was identified by two-dimensional electrophoresis. Silver-stained protein spots corresponding to the antigen were excised from two-dimensional electrophoresis gels, digested with trypsin, and analyzed by matrix-assisted laser desorption/ionization-reflectron time of flight and nano-electrospray ionization-ion trap/mass spectrometry. Data base search identified the HB antigen as the barrier-to-autointegration factor, a cellular protein implicated in the cellular cycle that blocks autointegration and promotes intermolecular integration of retrovirus such as the Moloney murine leukemia and the human immunodeficiency type 1 virus. The physicochemical characteristics described for these proteins, their ability to bind double-stranded DNA but not single-stranded DNA, and their nuclear localization confirm that HB and barrier-to-autointegration factor are the same protein.
|Journal||Journal of Biological Chemistry|
|Publication status||Published - 12 Dec 2003|
Forné, I., Carrascal, M., Martinez-Lostao, L., Abian, J., Rodriguez-Sánchez, J. L., & Juarez, C. (2003). Identification of the Autoantigen HB as the Barrier-to-Autointegration Factor. Journal of Biological Chemistry, 278(50), 50641-50644. https://doi.org/10.1074/jbc.M308531200