Identification of multicopy suppressors of cell cycle arrest at the G<inf>1</inf> - S transition in Saccharomyces cerevisiae

Ivan Muñoz, Ernesto Simón, Núria Casals, Josep Clotet, Joaquín Ariño

Research output: Contribution to journalReview articleResearchpeer-review

33 Citations (Scopus)

Abstract

Inactivation of HAL3 in the absence of SIT4 function leads to cell cycle arrest at the G1-S transition. To identify genes potentially involved in the control of this phase of the cell cycle, a screening for multicopy suppressors of a conditional sit4 hal3 mutant (strain JC002) has been developed. The screening yielded several genes known to perform key roles in cell cycle events, such as CLN3, BCK2 or SWI4, thus proving its usefulness as a tool for this type of studies. In addition, this approach allowed the identification of additional genes, most of them not previously related to the regulation of G1-S transition or even without known function (named here as VHS1-3, for viable in a hal3 sit4 background). Several of these gene products are involved in phosphodephosphorylation processes, including members of the protein phosphatase 2A and protein phosphatases 2C families, as well as components of the Hal5 protein kinase family. The ability of different genes to suppress sit4 phenotypes (such as temperature sensitivity and growth on non-fermentable carbon sources) or to mimic the functions of Hal3 was evaluated. The possible relationship between the known functions of these suppressor genes and the progress through the G1-S transition is discussed. Copyright © 2002 John Wiley & Sons, Ltd.
Original languageEnglish
Pages (from-to)157-169
JournalYeast
Volume20
Issue number2
DOIs
Publication statusPublished - 30 Jan 2003

Keywords

  • Cell cycle regulation
  • G - S transition 1
  • Protein kinases
  • Protein phosphatases
  • Saccharomyces cerevisiae

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