Identification of meiotic anomalies with multiplex fluorescence in situ hybridization: Preliminary results

Zaida Sarrate, Joan Blanco, Susana Egozcue, Francesca Vidal, Josep Egozcue

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To characterize meiotic anomalies in infertile men by multiplex fluorescence in situ hybridization (M-FISH) and to determine whether synaptic problems affect specific bivalents or whether anomalies are random. Analysis of meiotic preparations with standard techniques and M-FISH. Assisted reproduction centers and Universitat Autònoma de Barcelona. Three fertile men undergoing vasectomy, four sterile patients with oligoasthenoteratozoospermia, and one patient with a Robertsonian translocation t(13;14). Unilateral testicular biopsy in controls and patients with oligoasthenoteratozoospermia and collection of a semen sample from the translocation carrier. Identification of bivalents in metaphase I and chromosomes in metaphase II and characterization of chromosome abnormalities. All bivalents in metaphase I and all chromosomes in metaphase II could be identified. In controls and in one patient with oligoasthenoteratozoospermia, meiosis was normal. Other patients with oligoasthenoteratozoospermia showed different types of anomaly: desynapsis, breaks, precocious XY separation, or cryptic reorganizations. The Robertsonian translocation t(13;14) was easily identified. Results confirm the high incidence of synaptic errors in oligoasthenoteratozoospermia patients. Bivalents in metaphase I and chromosomes in metaphase II were individually identifiable. Nondisjunctional errors or small reorganizations overlooked in classic meiotic preparations were identified. Synaptic anomalies seem to affect meiotic bivalents at random. © 2004 by American Society for Reproductive Medicine.
Original languageEnglish
Pages (from-to)712-717
JournalFertility and Sterility
Publication statusPublished - 1 Sept 2004


  • Meiosis
  • chromosome reorganizations
  • multiplex FISH
  • synaptic anomalies


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