Identification of interleukin-27 (IL-27)/IL-27 receptor subunit alpha as a critical immune axis for in vivo HIV control

M. Ruiz-Riol, D. Berdnik, A. Llano, B. Mothe, C. Gálvez, S. Pérez-álvarez, B. Oriol-Tordera, A. Olvera, S. Silva-Arrieta, M. Meulbroek, F. Pujol, J. Coll, J. Martinez-Picado, C. Ganoza, J. Sanchez, G. Gómez, T. Wyss-Coray, C. Brander

    Research output: Contribution to journalArticleResearchpeer-review

    12 Citations (Scopus)


    © 2017 American Society for Microbiology. Intact and broad immune cell effector functions and specific individual cytokines have been linked to HIV disease outcome, but their relative contribution to HIV control remains unclear. We asked whether the proteome of secreted cytokines and signaling factors in peripheral blood can be used to discover specific pathways critical for host viral control. A custom glass-based microarray, able to measure >600 plasma proteins involved in cell-to-cell communication, was used to measure plasma protein profiles in 96 HIV-infected, treatment-naive individuals with high (>50,000) or low (<10,000 HIV RNA copies/ml) viral loads. Univariate and regression model analysis demonstrate that plasma levels of soluble interleukin-27 (IL- 27) are significantly elevated in individuals with high plasma viremia (P < 0.0001) and are positively correlated with proviral HIV-DNA copy numbers in peripheral blood mononuclear cells (PBMC) (Rho = 0.4011; P = 0.0027). Moreover, soluble IL-27 plasma levels are negatively associated with the breadth and magnitude of the total virus-specific T-cell responses and directly with plasma levels of molecules involved in Wnt/β-catenin signaling. In addition to IL-27, gene expression levels of the specific IL-27 receptor (IL27RA) in PBMC correlated directly with both plasma viral load (Rho = 0.3531; P = 0.0218) and the proviral copy number in the peripheral blood as an indirect measure of partial viral reservoir (Rho = 0.4580; P = 0.0030). These results were validated in unrelated cohorts of early infected subjects as well as subjects before and after initiation of antiretroviral treatment, and they identify IL-27 and its specific receptor as a critical immune axis for the antiviral immune response and as robust correlates of viral load and proviral reservoir size in PBMC.
    Original languageEnglish
    Article numbere00441-17
    JournalJournal of Virology
    Issue number16
    Publication statusPublished - 1 Aug 2017


    • Communicome
    • HIV
    • HIV replication control
    • IL-27 cytokine
    • Soluble communication factors
    • Th17 signaling
    • Wnt signaling


    Dive into the research topics of 'Identification of interleukin-27 (IL-27)/IL-27 receptor subunit alpha as a critical immune axis for in vivo HIV control'. Together they form a unique fingerprint.

    Cite this