Identification of genes involved in immune response of Atlantic salmon (Salmo salar) to IPN virus infection, using expressed sequence tag (EST) analysis

Victoria Cepeda, Catalina Cofre, Ruth González, Simon MacKenzie, Rodrigo Vidal

Research output: Contribution to journalArticleResearchpeer-review

13 Citations (Scopus)

Abstract

Atlantic salmon is one of the most important aquaculture fish species in the world. A cDNA library was constructed from splenic leukocytes of Atlantic salmon challenged with infectious pancreatic necrosis virus, and sequenced to collect genomic information and identify genes involved in immune defense response. Sequencing of 1360 clones yielded 1043 high quality ESTs (expressed sequence tags) and these ESTs were assembled into 823 unigenes. BLAST analysis revealed that 203 unigenes were novel genes that had no significant matches to any protein sequences in the public databases. The remaining 586 unigenes were closely matched to the known genes or sequences deposited in public databases and 34 unigenes matched to unknown genes. Among them, GO and KEGG analyses identified several functional categories involved in the innate immune response, with immune response and cytokine activity being predominant. These results provided a useful resource for gene discovery for further research of this species. © 2011 Elsevier B.V.
Original languageEnglish
Pages (from-to)54-60
JournalAquaculture
Volume318
Issue number1-2
DOIs
Publication statusPublished - 27 Jul 2011

Keywords

  • Atlantic salmon
  • CDNA library
  • Expressed sequence tag (EST)
  • Immune-related genes
  • Infectious pancreatic necrosis virus (IPNV)

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