Identification and characterization of inorganic-phosphate-solubilizing bacteria from agricultural fields with a rapid isolation method

Bang Xiao Zheng, Muhammad Ibrahim, Ding Peng Zhang, Qing Fang Bi, Hong Zhe Li, Guo Wei Zhou, Kai Ding, Josep Peñuelas, Yong Guan Zhu, Xiao Ru Yang

    Research output: Contribution to journalArticleResearch

    17 Citations (Scopus)

    Abstract

    © 2018, The Author(s). The ability to solubilize fixed inorganic phosphorus (P) for plant growth is important for increasing crop yield. More P can be released by inoculating soil with inorganic-phosphate-solubilizing bacteria (iPSBs). We used 96-well microplates instead of traditional 200-mm petri dishes to rapidly screen iPSB strains for their solubilizing ability. We simultaneously obtained 76 iPSB isolates from 576 wells containing two agricultural soils. This method conveniently identified positive iPSB strains and effectively prevented fungal cross-contamination. Maximum-likelihood phylogenetic trees of the isolated strains showed that Bacillus megaterium was the most dominant iPSB, and strains Y99, Y95, Y924 and Y1412 were selected as representatives for the analysis of P solubilization. Succinic acid was the main organic acid of B. megaterium for releasing P. It was strongly correlated with the increase in soluble P concentration during 168 h of incubation of these four strains. pH was negatively exponentially correlated with the amount of soluble P in the medium, and the amount of succinic acid was strongly linearly correlated with the amount of P released (P < 0.001), suggesting that organic acid may mobilize microbial P. Our study provides an efficient and effective method for identifying and analyzing the growth of iPSB strains able to solubilize inorganic P and gives a better understanding of the mechanism of P solubilization.
    Original languageEnglish
    Article number47
    JournalAMB Express
    Volume8
    DOIs
    Publication statusPublished - 1 Dec 2018

    Keywords

    • Characterization
    • Inorganic phosphate solubilizing bacteria
    • Isolation
    • Phosphorus

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