Hydrogen peroxide amperometric biosensor based on a peroxidase-graphite-epoxy biocomposite

A. Morales, F. Céspedes, J. Muñoz, E. Martínez-Fàbregas, S. Alegret

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51 Citations (Scopus)


An amperometric biosensor sensitive to hydrogen peroxide has been built, using a HRP-graphite-epoxy biocomposite. The proximity of the redox centers of the enzyme and the conductive sites at the surface of the electrode permits a direct regeneration of the enzyme. The response characteristics of this biosensor have been improved by adding powdered platinum to the matrix of the biocomposite. When Pt is absent, the working potential is -300 mV vs. Ag/AgCl and the response is linear between 0.03 mM and 7 mM. When 3% wt platinum is added to the biocomposite, a stable response is produced at -50 mV vs. Ag/AgCl and the linearity range lies between 0.09 mM and 9 mM. The response time is approximately 2 s for both the cases.
Original languageEnglish
Pages (from-to)131-138
JournalAnal. Chim. Acta
Issue number2-3
Publication statusPublished - 21 Oct 1996


  • Biosensors
  • Graphite-epoxy biocomposite
  • Horseradish peroxidase
  • Hydrogen peroxide


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