Hydrogen exchange monitored by MALDI-TOF mass spectrometry for rapid characterization of the stability and conformation of proteins

Josep Villanueva, Francesc Canals, Virtudes Villegas, Enrique Querol, Francesc X. Avilés

Research output: Contribution to journalArticleResearchpeer-review

24 Citations (Scopus)

Abstract

Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) has been used to monitor hydrogen exchange on entire proteins. Two alternative methods have been used to carry out the hydrogen exchange studies, exchanging deuteron (H to D experiments) or proton (D to H experiments). In the former case, the use of a deuterated matrix has made possible to overcome back-exchange problems and attain reproducible results. The methods presented have been used to determine the slow exchange core of the potato carboxypeptidase inhibitor in different folding states, and to differentially compare the activation domain of human procarboxypeptidase A2 versus three site-directed mutants of different conformational stability. In this work, we show that by using MALDI-TOF MS to monitor hydrogen exchange in entire proteins, it is possible to rapidly check the folding state of a protein and characterize mutational effects on protein conformation and stability, while requiring minimal amounts of sample. Copyright (C) 2000 Federation of European Biochemical Societies.
Original languageEnglish
Pages (from-to)27-33
JournalFEBS Letters
Volume472
DOIs
Publication statusPublished - 21 Apr 2000

Keywords

  • Activation domain of human procarboxypeptidase A2
  • Conformational stability
  • Hydrogen exchange
  • Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry
  • Potato carboxypeptidase inhibitor

Fingerprint

Dive into the research topics of 'Hydrogen exchange monitored by MALDI-TOF mass spectrometry for rapid characterization of the stability and conformation of proteins'. Together they form a unique fingerprint.

Cite this