Hybrid chloroperoxidase-magnetic nanoparticle clusters: effect of functionalization on biocatalyst performance

Gerard Masdeu, Slavko Kralj, Stane Pajk, Josep López-Santín, Darko Makovec, Gregorio Álvaro

Research output: Contribution to journalArticleResearchpeer-review

9 Citations (Scopus)

Abstract

© 2017 Society of Chemical Industry BACKGROUND: Hybrid enzyme–nanoparticle complexes, obtained by enzyme immobilization onto superparamagnetic particles, display unique properties for use as biocatalysts. A novel methodology for the immobilization of chloroperoxidase (CPO) onto magnetic nanoparticle clusters (mNC) is presented. Chloroperoxidase catalyzes alcohol oxidations using peroxides, and it has recently been shown to recognize β-amino alcohols as substrates, although high required peroxide concentration led to poor CPO stability. RESULTS: mNC retains the superparamagnetic properties of the single nanoparticle plus an increased magnetic moment, necessary for effective magnetic recovery. Different functional groups have been introduced on the silica layer that covers mNC. The linkage enzyme–support has been intended through different reactive groups on the CPO surface. The selected biocatalyst (95% yield, 63% retained activity), obtained by prior enzyme oxidation followed by coupling to the amino groups on the mNC surface, has been compared with soluble CPO in the model reaction (N-Cbz-3-aminopropanol oxidation) with significantly higher substrate conversion due to 4-fold increased enzyme stability. CONCLUSIONS: Functionalized mNC has demonstrated to be efficient for the preparation of hybrid enzyme-mNC biocatalysts. The systematic study of chloroperoxidase immobilization onto mNC led to several useful biocatalysts. The described methodology could easily be extended to many other enzymes in the preparation of efficient and reusable biocatalysts. © 2017 Society of Chemical Industry.
Original languageEnglish
Pages (from-to)233-245
JournalJournal of Chemical Technology and Biotechnology
Volume93
Issue number1
DOIs
Publication statusPublished - 1 Jan 2018

Keywords

  • amino alcohol oxidation
  • chloroperoxidase (CPO)
  • enzyme immobilization
  • enzyme stability
  • magnetic nanoparticle cluster

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