Humoral and in vivo cellular immunity against the raw insect-derived recombinant Leishmania infantum antigens KMPII, TRYP, LACK, and papLe22 in dogs from an endemic area

Felicitat Todolí, Laia Solano-Gallego, Rafael De Juan, Pere Morell, Maria Del Carmen Núñez, Rodrigo Lasa, Silvia Gómez-Sebastián, José M. Escribano, Jordi Alberola, Alhelí Rodríguez-Cortés

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6 Citations (Scopus)

Abstract

Leishmania infantum causes visceral leishmaniasis, a severe zoonotic and systemic disease that is fatal if left untreated. Identification of the antigens involved in Leishmania-specific protective immune response is a research priority for the development of effective control measures. For this purpose, we evaluated, in 27 dogs from an enzootic zone, specific humoral and cellular immune response by delayed-type hypersensitivity (DTH) skin test both against total L. infantum antigen and the raw Trichoplusia ni insect-derived kinetoplastid membrane protein-11 (rKMPII), tryparedoxin peroxidase (rTRYP), Leishmania homologue of receptors for activated C kinase (rLACK), and 22-kDa potentially aggravating protein of Leishmania (rpapLe22) antigens from this parasite. rTRYP induced the highest number of positive DTH responses (55% of leishmanin skin test [LST]-positive dogs), showing that TRYP antigen is an important T cell immunogen, and it could be a promising vaccine candidate against this disease. When TRYP-DTH and KMPII-DTH tests were evaluated in parallel, 82% of LST-positive dogs were detected, suggesting that both antigens could be considered as components of a standardized DTH immunodiagnostic tool for dogs. Copyright © 2010 by The American Society of Tropical Medicine and Hygiene.
Original languageEnglish
Pages (from-to)1287-1294
JournalAmerican Journal of Tropical Medicine and Hygiene
Volume83
DOIs
Publication statusPublished - 1 Dec 2010

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