TY - JOUR
T1 - Human papillomavirus and breast cancer: No evidence of association in a spanish set of cases
AU - Vernet-Tomas, Maria
AU - Mena, Marisa
AU - Alemany, Laia
AU - Bravo, Ignacio
AU - De Sanjosé, Silvia
AU - Nicolau, Pau
AU - Bergueiro, Alba
AU - Corominas, Josep Maria
AU - Serrano, Sergi
AU - Carreras, Ramon
AU - Lloveras, Belen
PY - 2015/1/1
Y1 - 2015/1/1
N2 - © 2015, International Institute of Anticancer Research. All rights reserved. Background/Aim: Great controversy exists about the association between Human Papillomavirus (HPV) and breast tumors. The aim of this study was to explore the presence of HPV DNA in a large set of breast cancer cases. Materials and Methods: Techniques used followed the standards for an international retrospective survey of HPV-DNA genotyping, coordinated by our own group and the DDL Laboratories in Rijswijk, the Netherlands. Paraffin-embedded samples were used. SPF-10 broad-spectrum primers were applied, followed by deoxyribonucleic acid enzyme immunoassay and genotyping by reverse-line probe assay. Results: A total of 78 samples were included in the study, 2 of benign conditions and 76 carcinomas, including different histological subtypes. HPV was not present in any of the specimens studied irrespective of histology, hormonal status and stage of disease. Conclusion: Our data do not support the involvement of HPV in breast carcinogenesis as no evidence of its presence was found.
AB - © 2015, International Institute of Anticancer Research. All rights reserved. Background/Aim: Great controversy exists about the association between Human Papillomavirus (HPV) and breast tumors. The aim of this study was to explore the presence of HPV DNA in a large set of breast cancer cases. Materials and Methods: Techniques used followed the standards for an international retrospective survey of HPV-DNA genotyping, coordinated by our own group and the DDL Laboratories in Rijswijk, the Netherlands. Paraffin-embedded samples were used. SPF-10 broad-spectrum primers were applied, followed by deoxyribonucleic acid enzyme immunoassay and genotyping by reverse-line probe assay. Results: A total of 78 samples were included in the study, 2 of benign conditions and 76 carcinomas, including different histological subtypes. HPV was not present in any of the specimens studied irrespective of histology, hormonal status and stage of disease. Conclusion: Our data do not support the involvement of HPV in breast carcinogenesis as no evidence of its presence was found.
KW - Breast cancer
KW - Human papillomavirus
KW - Polymerase chain reaction
M3 - Article
VL - 35
SP - 851
EP - 856
IS - 2
ER -
