TY - JOUR
T1 - Human origin of micronuclei in human × hamster two-cell embryos
AU - Tusell, Laura
AU - Alvarez, R.
AU - Genescà, A.
AU - Caballín, M. R.
AU - Miró, R.
AU - Barrios, L.
AU - Egozcue, J.
PY - 1995/1/1
Y1 - 1995/1/1
N2 - Using fluorescence in situ hybridization techniques with either human or hamster genomic DNA probes, we studied the origin of micronuclei in two-cell hybrid embryos obtained from hamster oocytes and γ-irradiated human spermatozoa. Our study demonstrates that over 99 % of micro nuclei hybridize with human DNA probes and not with hamster DNA, revealing their human origin. Thus, the micronucleus test represents a good method to evaluate genetic damage in human germ cells, since it is simpler and faster than sperm chromosome studies. © 1995 S. Karger AG, Basel.
AB - Using fluorescence in situ hybridization techniques with either human or hamster genomic DNA probes, we studied the origin of micronuclei in two-cell hybrid embryos obtained from hamster oocytes and γ-irradiated human spermatozoa. Our study demonstrates that over 99 % of micro nuclei hybridize with human DNA probes and not with hamster DNA, revealing their human origin. Thus, the micronucleus test represents a good method to evaluate genetic damage in human germ cells, since it is simpler and faster than sperm chromosome studies. © 1995 S. Karger AG, Basel.
U2 - https://doi.org/10.1159/000133987
DO - https://doi.org/10.1159/000133987
M3 - Article
VL - 70
SP - 41
EP - 44
JO - Cytogenetic and Genome Research
JF - Cytogenetic and Genome Research
SN - 1424-8581
ER -