Human intestinal αβ IEL clones in celiac disease show reduced IL-10 synthesis and enhanced IL-2 production

Edgardo C. Kolkowski, Marco A. Fernández, Ricardo Pujol-Borrell, Dolores Jaraquemada

Research output: Contribution to journalArticleResearchpeer-review

8 Citations (Scopus)

Abstract

Celiac disease is a gluten-induced T-cell mediated autoimmune process that results in the destruction of the intestinal mucosa and is associated with an expansion of CD8+ CD103+ TCRαβ intraepithelial lymphocytes (IELs) in the damaged epithelium. The role of this IEL population in the pathology is unknown. The aim of this work was to compare the cytokine profile and the cytotoxicity pattern from CD8+ IEL clones isolated from celiac (CD) and non-celiac (NCD) biopsies. We report that the number of IL-10 producing CD clones was significantly lower (26%) than that obtained from the NCD sample (62%). Instead, IL-2 was produced by more CD (44%) than NCD clones (26%). Cytotoxicity patterns against intestinal epithelial cell lines suggest different functional subsets of CD8+ IELs. CD clones capable of high cytotoxicity produced IL-2 whereas most cytotoxic NCD IELs produced IL-10. This clonal analysis indicates that an impaired immune regulation in celiac mucosa may be partially attributed to the low generation of regulatory CD8+ IELs that produce IL-10. © 2007 Elsevier Inc. All rights reserved.
Original languageEnglish
Pages (from-to)1-9
JournalCellular Immunology
Volume244
Issue number1
DOIs
Publication statusPublished - 1 Nov 2006

Keywords

  • CBA (Cytometric bead array)
  • Celiac disease
  • IEL (Intraepithelial lymphocytes)
  • IL-10
  • IL-2
  • TCRαβ

Fingerprint Dive into the research topics of 'Human intestinal αβ IEL clones in celiac disease show reduced IL-10 synthesis and enhanced IL-2 production'. Together they form a unique fingerprint.

Cite this