HPLC quantification of perphenazine in sheep plasma:: Application to a pharmacokinetic study

Cecilia Pedernera Romano; José Luis Ruiz de la Torre Casañas; Glòria Castells Carles; Francisco Javier Manteca Vilanova; Carles Cristòfol Adell

doi:10.1016/j.jchromb.2007.04.038

HPLC quantification of perphenazine in sheep plasma: Application to a pharmacokinetic study

Cecilia Pedernera Romano, José Luis Ruiz de la Torre Casañas, Glòria Castells Carles, Francisco Javier Manteca Vilanova, Carles Cristòfol Adell

Research output: Contribution to journal › Article › Research › peer-review

12 Citations (Scopus)

Abstract

A high performance liquid chromatographic (HPLC) method for the determination of perphenazine (PPZ) in sheep plasma was developed and validated. The separation was achieved using a 5 μm C18 column (125 mm × 4 mm) with a mobile phase composed of acetonitrile and an aqueous solution of H3PO4 and TBA (inverse gradient). The flow rate was 1.5 mL/min and the UV detection was performed at 258 nm. The method was validated with respect to linearity, intra and inter-day precision and accuracy, limit of quantification, limit of detection and storage stability. This method was used to perform a pilot pharmacokinetic study of PPZ after subcutaneous administration to one ewe.

Original language	English
Pages (from-to)	308-312
Number of pages	5
Journal	Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Volume	854
Issue number	1-2
DOIs	https://doi.org/10.1016/j.jchromb.2007.04.038
Publication status	Published - Jul 2007

Keywords

Perphenazine
HPLC
UV
Pharmacokinetic
Sheep

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10.1016/j.jchromb.2007.04.038Licence: C In Copyright

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Pedernera Romano, C., Ruiz de la Torre Casañas, J. L., Castells Carles, G., Manteca Vilanova, F. J., & Cristòfol Adell, C. (2007). HPLC quantification of perphenazine in sheep plasma: Application to a pharmacokinetic study. Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, 854(1-2), 308-312. https://doi.org/10.1016/j.jchromb.2007.04.038

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title = "HPLC quantification of perphenazine in sheep plasma:: Application to a pharmacokinetic study",

abstract = "A high performance liquid chromatographic (HPLC) method for the determination of perphenazine (PPZ) in sheep plasma was developed and validated. The separation was achieved using a 5 μm C18 column (125 mm × 4 mm) with a mobile phase composed of acetonitrile and an aqueous solution of H3PO4 and TBA (inverse gradient). The flow rate was 1.5 mL/min and the UV detection was performed at 258 nm. The method was validated with respect to linearity, intra and inter-day precision and accuracy, limit of quantification, limit of detection and storage stability. This method was used to perform a pilot pharmacokinetic study of PPZ after subcutaneous administration to one ewe.",

keywords = "Perphenazine, HPLC, UV, Pharmacokinetic, Sheep",

author = "{Pedernera Romano}, Cecilia and {Ruiz de la Torre Casa{\~n}as}, {Jos{\'e} Luis} and {Castells Carles}, Gl{\`o}ria and {Manteca Vilanova}, {Francisco Javier} and {Crist{\`o}fol Adell}, Carles",

year = "2007",

month = jul,

doi = "10.1016/j.jchromb.2007.04.038",

language = "English",

volume = "854",

pages = "308--312",

number = "1-2",

}

Pedernera Romano, C, Ruiz de la Torre Casañas, JL, Castells Carles, G , Manteca Vilanova, FJ & Cristòfol Adell, C 2007, 'HPLC quantification of perphenazine in sheep plasma: Application to a pharmacokinetic study', Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, vol. 854, no. 1-2, pp. 308-312. https://doi.org/10.1016/j.jchromb.2007.04.038

HPLC quantification of perphenazine in sheep plasma: Application to a pharmacokinetic study. / Pedernera Romano, Cecilia; Ruiz de la Torre Casañas, José Luis; Castells Carles, Glòria et al.
In: Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, Vol. 854, No. 1-2, 07.2007, p. 308-312.

Research output: Contribution to journal › Article › Research › peer-review

TY - JOUR

T1 - HPLC quantification of perphenazine in sheep plasma:

T2 - Application to a pharmacokinetic study

AU - Pedernera Romano, Cecilia

AU - Ruiz de la Torre Casañas, José Luis

AU - Castells Carles, Glòria

AU - Manteca Vilanova, Francisco Javier

AU - Cristòfol Adell, Carles

PY - 2007/7

Y1 - 2007/7

N2 - A high performance liquid chromatographic (HPLC) method for the determination of perphenazine (PPZ) in sheep plasma was developed and validated. The separation was achieved using a 5 μm C18 column (125 mm × 4 mm) with a mobile phase composed of acetonitrile and an aqueous solution of H3PO4 and TBA (inverse gradient). The flow rate was 1.5 mL/min and the UV detection was performed at 258 nm. The method was validated with respect to linearity, intra and inter-day precision and accuracy, limit of quantification, limit of detection and storage stability. This method was used to perform a pilot pharmacokinetic study of PPZ after subcutaneous administration to one ewe.

AB - A high performance liquid chromatographic (HPLC) method for the determination of perphenazine (PPZ) in sheep plasma was developed and validated. The separation was achieved using a 5 μm C18 column (125 mm × 4 mm) with a mobile phase composed of acetonitrile and an aqueous solution of H3PO4 and TBA (inverse gradient). The flow rate was 1.5 mL/min and the UV detection was performed at 258 nm. The method was validated with respect to linearity, intra and inter-day precision and accuracy, limit of quantification, limit of detection and storage stability. This method was used to perform a pilot pharmacokinetic study of PPZ after subcutaneous administration to one ewe.

KW - Perphenazine

KW - HPLC

KW - UV

KW - Pharmacokinetic

KW - Sheep

U2 - 10.1016/j.jchromb.2007.04.038

DO - 10.1016/j.jchromb.2007.04.038

M3 - Article

SN - 1570-0232

VL - 854

SP - 308

EP - 312

JO - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences

JF - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences

IS - 1-2

ER -

HPLC quantification of perphenazine in sheep plasma: Application to a pharmacokinetic study

Abstract

Keywords

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