Abstract
A high performance liquid chromatographic (HPLC) method for the determination of perphenazine (PPZ) in sheep plasma was developed and validated. The separation was achieved using a 5 μm C18 column (125 mm × 4 mm) with a mobile phase composed of acetonitrile and an aqueous solution of H3PO4 and TBA (inverse gradient). The flow rate was 1.5 mL/min and the UV detection was performed at 258 nm. The method was validated with respect to linearity, intra and inter-day precision and accuracy, limit of quantification, limit of detection and storage stability. This method was used to perform a pilot pharmacokinetic study of PPZ after subcutaneous administration to one ewe. © 2007 Elsevier B.V. All rights reserved.
Original language | English |
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Pages (from-to) | 308-312 |
Journal | Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences |
Volume | 854 |
DOIs | |
Publication status | Published - 1 Jul 2007 |
Keywords
- HPLC
- Perphenazine
- Pharmacokinetic
- Sheep
- UV