HPLC quantification of perphenazine in sheep plasma: Application to a pharmacokinetic study

Cecilia Pedernera; José Luis Ruiz; Glòria Castells; Xavier Manteca; Carles Cristòfol

doi:https://doi.org/10.1016/j.jchromb.2007.04.038

HPLC quantification of perphenazine in sheep plasma: Application to a pharmacokinetic study

Cecilia Pedernera, José Luis Ruiz, Glòria Castells, Xavier Manteca, Carles Cristòfol

Research output: Contribution to journal › Article › Research › peer-review

11 Citations (Scopus)

Abstract

A high performance liquid chromatographic (HPLC) method for the determination of perphenazine (PPZ) in sheep plasma was developed and validated. The separation was achieved using a 5 μm C18 column (125 mm × 4 mm) with a mobile phase composed of acetonitrile and an aqueous solution of H3PO4 and TBA (inverse gradient). The flow rate was 1.5 mL/min and the UV detection was performed at 258 nm. The method was validated with respect to linearity, intra and inter-day precision and accuracy, limit of quantification, limit of detection and storage stability. This method was used to perform a pilot pharmacokinetic study of PPZ after subcutaneous administration to one ewe. © 2007 Elsevier B.V. All rights reserved.

Original language	English
Pages (from-to)	308-312
Journal	Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Volume	854
DOIs	https://doi.org/10.1016/j.jchromb.2007.04.038
Publication status	Published - 1 Jul 2007

Keywords

HPLC
Perphenazine
Pharmacokinetic
Sheep
UV

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https://doi.org/10.1016/j.jchromb.2007.04.038

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title = "HPLC quantification of perphenazine in sheep plasma: Application to a pharmacokinetic study",

abstract = "A high performance liquid chromatographic (HPLC) method for the determination of perphenazine (PPZ) in sheep plasma was developed and validated. The separation was achieved using a 5 μm C18 column (125 mm × 4 mm) with a mobile phase composed of acetonitrile and an aqueous solution of H3PO4 and TBA (inverse gradient). The flow rate was 1.5 mL/min and the UV detection was performed at 258 nm. The method was validated with respect to linearity, intra and inter-day precision and accuracy, limit of quantification, limit of detection and storage stability. This method was used to perform a pilot pharmacokinetic study of PPZ after subcutaneous administration to one ewe. {\textcopyright} 2007 Elsevier B.V. All rights reserved.",

keywords = "HPLC, Perphenazine, Pharmacokinetic, Sheep, UV",

author = "Cecilia Pedernera and Ruiz, {Jos{\'e} Luis} and Gl{\`o}ria Castells and Xavier Manteca and Carles Crist{\`o}fol",

year = "2007",

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doi = "https://doi.org/10.1016/j.jchromb.2007.04.038",

language = "English",

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HPLC quantification of perphenazine in sheep plasma: Application to a pharmacokinetic study. / Pedernera, Cecilia; Ruiz, José Luis; Castells, Glòria; Manteca, Xavier ; Cristòfol, Carles.

In: Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, Vol. 854, 01.07.2007, p. 308-312.

Research output: Contribution to journal › Article › Research › peer-review

TY - JOUR

T1 - HPLC quantification of perphenazine in sheep plasma: Application to a pharmacokinetic study

AU - Pedernera, Cecilia

AU - Ruiz, José Luis

AU - Castells, Glòria

AU - Manteca, Xavier

AU - Cristòfol, Carles

PY - 2007/7/1

Y1 - 2007/7/1

N2 - A high performance liquid chromatographic (HPLC) method for the determination of perphenazine (PPZ) in sheep plasma was developed and validated. The separation was achieved using a 5 μm C18 column (125 mm × 4 mm) with a mobile phase composed of acetonitrile and an aqueous solution of H3PO4 and TBA (inverse gradient). The flow rate was 1.5 mL/min and the UV detection was performed at 258 nm. The method was validated with respect to linearity, intra and inter-day precision and accuracy, limit of quantification, limit of detection and storage stability. This method was used to perform a pilot pharmacokinetic study of PPZ after subcutaneous administration to one ewe. © 2007 Elsevier B.V. All rights reserved.

AB - A high performance liquid chromatographic (HPLC) method for the determination of perphenazine (PPZ) in sheep plasma was developed and validated. The separation was achieved using a 5 μm C18 column (125 mm × 4 mm) with a mobile phase composed of acetonitrile and an aqueous solution of H3PO4 and TBA (inverse gradient). The flow rate was 1.5 mL/min and the UV detection was performed at 258 nm. The method was validated with respect to linearity, intra and inter-day precision and accuracy, limit of quantification, limit of detection and storage stability. This method was used to perform a pilot pharmacokinetic study of PPZ after subcutaneous administration to one ewe. © 2007 Elsevier B.V. All rights reserved.

KW - HPLC

KW - Perphenazine

KW - Pharmacokinetic

KW - Sheep

KW - UV

U2 - https://doi.org/10.1016/j.jchromb.2007.04.038

DO - https://doi.org/10.1016/j.jchromb.2007.04.038

M3 - Article

VL - 854

SP - 308

EP - 312

JO - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences

JF - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences

SN - 1570-0232

ER -

HPLC quantification of perphenazine in sheep plasma: Application to a pharmacokinetic study

Abstract

Keywords

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