Homomeric Kv7.2 current suppression is a common feature in KCNQ2 epileptic encephalopathy

Carolina Gomis-Pérez, Janire Urrutia, Anna Marcé-Grau, Covadonga Malo, Eduardo López-Laso, Ana Felipe-Rucián, Miquel Raspall-Chaure, Alfons Macaya, Alvaro Villarroel

Research output: Contribution to journalArticleResearch

4 Citations (Scopus)

Abstract

Wiley Periodicals, Inc. © 2018 International League Against Epilepsy Objective: To gain insight into the mechanisms underlying KCNQ2 encephalopathy by examining the electrophysiologic properties of mutant Kv7.2 channels in different multimeric configurations. Methods: We analyzed the genotype-phenotype relationship in 4 patients with KCNQ2 encephalopathy and performed electrophysiologic analysis of M-currents mediated by homomeric Kv7.2 or heteromeric Kv7.2/Kv7.3 channels. Results: Negligible or no current was recorded in cells expressing homomeric E130K, W270R, or G281R de novo mutants, and it was reduced by more than 90% for the L243F maternally inherited mutant. The E130K and G281R mutants presented a marked dominant-negative behavior, whereas the current density was partially reduced (L243F) or not affected (W270R) when coexpressed with wild-type Kv7.2 subunits. In contrast, the extent of Kv7.3 “rescue,” which yields negligible currents on its own, followed the sequence E130K > L243F > W270R, whereas no rescue was observed with the G281R mutant. No significant effects on current density were observed when subunits were expressed in a 0.5:0.5:1.0 (Kv7.2:mutant:Kv7.3) DNA ratio to mimic the genetic balance. There was an increase in sensitivity to phosphatidylinositol 4,5-bisphosphate (PIP 2 ) depletion for W270R/Kv7.3, but no substantial differences were observed when the mutated subunits were coexpressed with Kv7.2 or both Kv7.2 and Kv7.3. Significance: There was a marked disparity of the impact of these mutations on Kv7.2 function, which varied on association with Kv7.2 or Kv7.3 subunits. Current density of homomeric channels was the most reliable property relating Kv7.2 function to encephalopathy, but other factors are required to explain the milder phenotype for some individuals carrying the maternally inherited L243F mutation. We hypothesize that the role of homomeric Kv7.2 channels for fine-tuning neuronal connections during development is critical for the severity of the KCNQ2 encephalopathy.
Original languageEnglish
Pages (from-to)139-148
JournalEpilepsia
Volume60
DOIs
Publication statusPublished - 1 Jan 2019

Keywords

  • early infantile epileptic encephalopathies
  • epilepsy
  • KCNQ2
  • M-current
  • PIP 2

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