HLADH-catalyzed synthesis of β-amino acids, assisted by continuous electrochemical regeneration of NAD+ in a filter press microreactor

Rossmery A. Rodríguez-Hinestroza, Carmen López, Josep López-Santín, Cheikhou Kane, M. Dolors Benaiges, Theo Tzedakis

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5 Citations (Scopus)

Abstract

© 2016 Elsevier Ltd This work focuses on the use of electrochemical microreactors, applied to the direct electroregeneration of nicotinamide dinucleotide (NAD+) used in-situ for the enzymatically-assisted oxidation of the β-alanine. Mechanistic and chemical/electrochemical reactor approaches were investigated, to achieve satisfactory conversions (>80%) of the substrate in reasonable (1–2 days) reaction times; no fouling observed of the gold anode. An original method was proposed to limit the complexation effect of the produced β-alanine to the enzyme. Simulations of the overall system were achieved and an estimation of the required kinetic and Michaelis constants was proposed.
Original languageEnglish
Pages (from-to)196-207
JournalChemical Engineering Science
Volume158
DOIs
Publication statusPublished - 2 Feb 2017

Keywords

  • Electrochemical NAD regeneration +
  • Electrochemical microreactor
  • Horse-liver alcohol dehydrogenase
  • β-alanine synthesis, simulation
  • β-amino acids

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