HLADH-catalyzed synthesis of β-amino acids, assisted by continuous electrochemical regeneration of NAD+ in a filter press microreactor

Rossmery A. Rodríguez-Hinestroza; Carmen López; Josep López-Santín; Cheikhou Kane; M. Dolors Benaiges; Theo Tzedakis

doi:https://doi.org/10.1016/j.ces.2016.10.010

HLADH-catalyzed synthesis of β-amino acids, assisted by continuous electrochemical regeneration of NAD⁺ in a filter press microreactor

Rossmery A. Rodríguez-Hinestroza, Carmen López, Josep López-Santín, Cheikhou Kane, M. Dolors Benaiges, Theo Tzedakis

Department of Chemical, Biological and Environmental Engineering

Research output: Contribution to journal › Article › Research › peer-review

5 Citations (Scopus)

Abstract

© 2016 Elsevier Ltd This work focuses on the use of electrochemical microreactors, applied to the direct electroregeneration of nicotinamide dinucleotide (NAD+) used in-situ for the enzymatically-assisted oxidation of the β-alanine. Mechanistic and chemical/electrochemical reactor approaches were investigated, to achieve satisfactory conversions (>80%) of the substrate in reasonable (1–2 days) reaction times; no fouling observed of the gold anode. An original method was proposed to limit the complexation effect of the produced β-alanine to the enzyme. Simulations of the overall system were achieved and an estimation of the required kinetic and Michaelis constants was proposed.

Original language	English
Pages (from-to)	196-207
Journal	Chemical Engineering Science
Volume	158
DOIs	https://doi.org/10.1016/j.ces.2016.10.010
Publication status	Published - 2 Feb 2017

Keywords

Electrochemical NAD regeneration +
Electrochemical microreactor
Horse-liver alcohol dehydrogenase
β-alanine synthesis, simulation
β-amino acids

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https://doi.org/10.1016/j.ces.2016.10.010

Cite this

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title = "HLADH-catalyzed synthesis of β-amino acids, assisted by continuous electrochemical regeneration of NAD+ in a filter press microreactor",

abstract = "{\textcopyright} 2016 Elsevier Ltd This work focuses on the use of electrochemical microreactors, applied to the direct electroregeneration of nicotinamide dinucleotide (NAD+) used in-situ for the enzymatically-assisted oxidation of the β-alanine. Mechanistic and chemical/electrochemical reactor approaches were investigated, to achieve satisfactory conversions (>80%) of the substrate in reasonable (1–2 days) reaction times; no fouling observed of the gold anode. An original method was proposed to limit the complexation effect of the produced β-alanine to the enzyme. Simulations of the overall system were achieved and an estimation of the required kinetic and Michaelis constants was proposed.",

keywords = "Electrochemical NAD regeneration +, Electrochemical microreactor, Horse-liver alcohol dehydrogenase, β-alanine synthesis, simulation, β-amino acids",

author = "Rodr{\'i}guez-Hinestroza, {Rossmery A.} and Carmen L{\'o}pez and Josep L{\'o}pez-Sant{\'i}n and Cheikhou Kane and Benaiges, {M. Dolors} and Theo Tzedakis",

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doi = "https://doi.org/10.1016/j.ces.2016.10.010",

language = "English",

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journal = "Chemical Engineering Science",

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HLADH-catalyzed synthesis of β-amino acids, assisted by continuous electrochemical regeneration of NAD⁺ in a filter press microreactor. / Rodríguez-Hinestroza, Rossmery A.; López, Carmen; López-Santín, Josep; Kane, Cheikhou; Benaiges, M. Dolors; Tzedakis, Theo.

In: Chemical Engineering Science, Vol. 158, 02.02.2017, p. 196-207.

Research output: Contribution to journal › Article › Research › peer-review

TY - JOUR

T1 - HLADH-catalyzed synthesis of β-amino acids, assisted by continuous electrochemical regeneration of NAD+ in a filter press microreactor

AU - Rodríguez-Hinestroza, Rossmery A.

AU - López, Carmen

AU - López-Santín, Josep

AU - Kane, Cheikhou

AU - Benaiges, M. Dolors

AU - Tzedakis, Theo

PY - 2017/2/2

Y1 - 2017/2/2

N2 - © 2016 Elsevier Ltd This work focuses on the use of electrochemical microreactors, applied to the direct electroregeneration of nicotinamide dinucleotide (NAD+) used in-situ for the enzymatically-assisted oxidation of the β-alanine. Mechanistic and chemical/electrochemical reactor approaches were investigated, to achieve satisfactory conversions (>80%) of the substrate in reasonable (1–2 days) reaction times; no fouling observed of the gold anode. An original method was proposed to limit the complexation effect of the produced β-alanine to the enzyme. Simulations of the overall system were achieved and an estimation of the required kinetic and Michaelis constants was proposed.

AB - © 2016 Elsevier Ltd This work focuses on the use of electrochemical microreactors, applied to the direct electroregeneration of nicotinamide dinucleotide (NAD+) used in-situ for the enzymatically-assisted oxidation of the β-alanine. Mechanistic and chemical/electrochemical reactor approaches were investigated, to achieve satisfactory conversions (>80%) of the substrate in reasonable (1–2 days) reaction times; no fouling observed of the gold anode. An original method was proposed to limit the complexation effect of the produced β-alanine to the enzyme. Simulations of the overall system were achieved and an estimation of the required kinetic and Michaelis constants was proposed.

KW - Electrochemical NAD regeneration +

KW - Electrochemical microreactor

KW - Horse-liver alcohol dehydrogenase

KW - β-alanine synthesis, simulation

KW - β-amino acids

U2 - https://doi.org/10.1016/j.ces.2016.10.010

DO - https://doi.org/10.1016/j.ces.2016.10.010

M3 - Article

VL - 158

SP - 196

EP - 207

JO - Chemical Engineering Science

JF - Chemical Engineering Science

SN - 0009-2509