Abstract
© 2016 Elsevier Ltd This work focuses on the use of electrochemical microreactors, applied to the direct electroregeneration of nicotinamide dinucleotide (NAD+) used in-situ for the enzymatically-assisted oxidation of the β-alanine. Mechanistic and chemical/electrochemical reactor approaches were investigated, to achieve satisfactory conversions (>80%) of the substrate in reasonable (1–2 days) reaction times; no fouling observed of the gold anode. An original method was proposed to limit the complexation effect of the produced β-alanine to the enzyme. Simulations of the overall system were achieved and an estimation of the required kinetic and Michaelis constants was proposed.
Original language | English |
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Pages (from-to) | 196-207 |
Journal | Chemical Engineering Science |
Volume | 158 |
DOIs | |
Publication status | Published - 2 Feb 2017 |
Keywords
- Electrochemical NAD regeneration +
- Electrochemical microreactor
- Horse-liver alcohol dehydrogenase
- β-alanine synthesis, simulation
- β-amino acids