Hla DR, DP, DQ induction in human islet β cells by the cytokine combination IFN-γ + TNF-α

Gloria Soldevila, Mala Doshi, Roger James, Stephen P. Lake, Robert Sutton, Derek Gray, Gian Franco Bottazzo, Ricardo Pujol-Borrell

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    5 Citations (Scopus)

    Abstract

    Human islet β cells do not express HLA Class II normally, yet, in the diabetic pancreas, β cells are selectively positive for Class II and this may facilitate their recognition by T cells. It has been demonstrated that human β cells can be induced to express Class II when cultured with IFN-γ + TNF-α or IFN-γ + TNF-β To assess whether or not they can be induced to express the products of the Class II subregions, DR, DP and DQ, human islet cultures from 10 pancreases were supplemented with the combination of IFN-γ + TNF-α using MoAbs specific for DR, DP and DQ products, and antibodies to insulin and glucagon. The combination IFN-γ + TNF-α (100-1000 U/ml each) was able to induce the expression of the three subregions in both β and α cells. The induction of subregion expression followed the hierarchy DR > DQ ≥ DP. The capability of β cells to express all three Class II subregions supports the possibility that these cells can present their self antigens to T cells. © 1990 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted.
    Original languageEnglish
    Pages (from-to)307-317
    JournalAutoimmunity
    Volume6
    Issue number4
    DOIs
    Publication statusPublished - 1 Jan 1990

    Keywords

    • HLA-Classs II subregion
    • Type I diabetes
    • β cells

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