Histopatological validation of tissue-microarray technology in urotelial cancer. Our experience

A. Laborda Rodríguez, F. Vallmanya Llena, R. Cortadellas Angel, J. Lloreta Trull, A. Gelabert Mas

Research output: Contribution to journalArticleResearchpeer-review

2 Citations (Scopus)

Abstract

Introduction: The array technology offers: a big advance to clinic and basic investigator, it provides a variety of technics (immunohystochemistry, FISH, proteomics) to undestand the molecular mechanisms of cancer. It offers scale economy in reagents versus the conventional methods. Array most be ratified because the sample is so reduced. Material and Methods: 52 consecutive cases have been cloosen from paraffin blocks of bladder and ureteral cancer which are 5-7 years old, a tissue array has been made; disks have been arranged in lines and columns, in an aleatory way, in order to guide it's reading. It has been evaluated by a pathologist with any relation to specimen selection. Results: 87 sheets ha been obtained. Number 1 has been dyed with HE. Has been discrepancy in 27% of sample's stage. Has not been a discrepancy in hystopathologic diagnostic. There is no sample's representation in 11 points (17%). Discussion: Our results offer good results in sample's validation. The sample's antigenicity of tissue is conserved. Array sample's represent a 97%, similary to all unit of conventional sections of the specimen.
Original languageEnglish
Pages (from-to)215-220
JournalActas Urologicas Espanolas
Volume28
Issue number3
DOIs
Publication statusPublished - 1 Jan 2004

Keywords

  • Bladder cancer
  • FISH
  • Immunohistochemistry
  • Microarray
  • Renal pelvis cancer

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