Histone H1 subtype synthesis in neurons and neuroblasts

Valentín Domínguez, Benjamín Piña, Pedro Suau

Research output: Contribution to journalArticleResearchpeer-review

36 Citations (Scopus)


Rat cerebral cortex neurons contain the five histone H1 subtypes H1a-e and the subtype H1° present in other mammalian somatic tissues. The four subtypes H1a-d decay exponentially during postnatal development and are partially or totally replaced by H1e that becomes the major H1 subtype in adults. H1° accumulates in a period restricted to neuronal terminal differentiation. Here we study the synthesis of the H1 subtypes in cortical neurons and their neuroblasts by in vivo labeling with [14C]lysine. The subtype synthesis pattern of neuroblasts has been determined by labeling gravid rats during the period of proliferation of cortical neurons and synthesis in neurons has been studied by postnatal labeling. The subtype H1a is synthesized in neuroblasts but not in neurons and is therefore rapidly removed from neuronal chromatin. The synthesis of H1b and Hid is much lower in neurons than in neuroblasts so that these subtypes are replaced to a large extent during postnatal development. H1c is synthesized at levels much higher than the other subtypes both in neurons and neuroblasts, but its very high turnover, about one order of magnitude faster than that of H1e in neurons, favors its partial replacement during postnatal development. Comparison of the synthesis rates of H1° in newborn and 30-day-old rats shows that the accumulation of H1° in differentiating neurons is due to an increased level of synthesis.
Original languageEnglish
Pages (from-to)181-185
JournalDevelopment (Cambridge)
Issue number1
Publication statusPublished - 1 May 1992


  • Differentiation
  • Histone H1
  • Neuroblast
  • Neuron
  • Subtype


Dive into the research topics of 'Histone H1 subtype synthesis in neurons and neuroblasts'. Together they form a unique fingerprint.

Cite this