Histological and Cytological Techniques to Study Perinatal Mouse Ovaries and Oocytes

Nikoleta Nikou, Maria López Panadés, Ignasi Roig*

*Corresponding author for this work

Research output: Chapter in BookChapterResearchpeer-review

Abstract

The regulation of female fertility in mammals depends on critical processes during oocyte development and maturation. Therefore, it is crucial to use specific approaches when studying mammalian female fertility to preserve ovary and oocyte structures effectively. The methods of collecting and culturing ovaries and oocytes play an essential role in the study of mammalian follicle development and oocyte quality. This chapter presents a collection of protocols that focus on various methods for studying mammalian ovaries and oocytes, providing researchers with a variety of approaches to choose from.

Original languageEnglish
Title of host publicationGerm Cell Development
Subtitle of host publicationMeethoids and protocols
Place of PublicationNew York
Chapter12
Pages151-170
Number of pages20
Volume2770
ISBN (Electronic)978-1-0716-3698-5
DOIs
Publication statusPublished - 15 Feb 2024

Publication series

NameMethods in Molecular Biology
Volume2770

Keywords

  • Culture
  • Histology
  • Immunofluorescence
  • Mammalian oocytes
  • Mammalian ovaries

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