High-throughput, functional screening of the anti-HIV-1 humoral response by an enzymatic nanosensor

Rosa María Ferraz, Anna Arís, Miguel Angel Martínez, Antonio Villaverde

Research output: Contribution to journalArticleResearchpeer-review

13 Citations (Scopus)

Abstract

The impact of antibodies on the target's epitope conformation is a major determinant of HIV-1 neutralization and a potential contributor to disease progression. We explore here a conformation-sensitive enzymatic nanosensor for the high-throughput functional screening of human anti-HIV-1 antibodies in sera. When displaying a model epitope from a gp41 immunodominant region (Env residues from 579 to 613), the sensing signal quantitatively distinguishes between adaptive and non-adaptive antibody binding. By using this tool, we have identified IgG4 as the immunoglobulin subpopulation most efficient in the structural modification of the target epitope. © 2006 Elsevier Ltd. All rights reserved.
Original languageEnglish
Pages (from-to)2119-2123
JournalMolecular Immunology
Volume43
Issue number13
DOIs
Publication statusPublished - 1 May 2006

Keywords

  • Adaptive binding
  • Allosteric biosensor
  • Antibody
  • HIV
  • IgG4
  • Immunosensor
  • Viral infection

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