High resolution microdialysis. Methodological issues and application to the study of inflammatory brain response

J. A. De Los Rios, Juan Sahuquillo, M. A. Merino, M. A. Poca, L. Expósito

    Research output: Contribution to journalReview articleResearchpeer-review

    5 Citations (Scopus)

    Abstract

    Cerebral microdialysis is a tool that provides very relevant information in the metabolic monitoring of brain injured patients. It is a particularly effective technique for the detection and analysis of small molecules, given that the pores of the dialysis membrane act as a barrier to restrict the transport of larger species, such as proteins and other macromolecules. The recent availability of microdialysis catheters with membrane pores of larger size, termed "high resolution" catheters, would widen the spectrum of molecules detectable in the dialisate. However, there are technical complications related to the use of these catheters for such purposes, and therefore, this potential capacity for the recovery of proteins needs to be validated, in order to begin its application as a tool in studies of proteomics associated with brain injuries. The following review depicts the basic principles of microdialysis, and describes some of the issues involved in the recovery of molecules in the dialisate, including the physical properties of the dialysis membrane and of the molecules of interest.
    Original languageEnglish
    Pages (from-to)433-447
    JournalNeurocirugia
    Volume20
    Issue number5
    Publication statusPublished - 1 Jan 2009

    Keywords

    • Chemokines
    • Cytokines
    • Membrane cut off
    • Microdialysis
    • Neuroinflammation
    • Relative recovery
    • Traumatic brain injury

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