High pressure inertial focusing for separating and concentrating bacteria at high throughput

J. Cruz, S. Hooshmand Zadeh, T. Graells, M. Andersson, J. Malmström, Z. G. Wu, K. Hjort

Research output: Contribution to journalArticleResearchpeer-review

8 Citations (Scopus)

Abstract

© 2017 IOP Publishing Ltd. Inertial focusing is a promising microfluidic technology for concentration and separation of particles by size. However, there is a strong correlation of increased pressure with decreased particle size. Theory and experimental results for larger particles were used to scale down the phenomenon and find the conditions that focus 1 μm particles. High pressure experiments in robust glass chips were used to demonstrate the alignment. We show how the technique works for 1 μm spherical polystyrene particles and for Escherichia coli, not being harmful for the bacteria at 50 μl min -1 . The potential to focus bacteria, simplicity of use and high throughput make this technology interesting for healthcare applications, where concentration and purification of a sample may be required as an initial step.
Original languageEnglish
Article number084001
JournalJournal of Micromechanics and Microengineering
Volume27
Issue number8
DOIs
Publication statusPublished - 27 Jun 2017

Keywords

  • PDMS
  • bacteria separation
  • glass chips
  • high pressure
  • inertial focusing
  • microfluidic channel
  • particle separation

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