High-performance liquid chromatographic separation of caffeine, theophylline, theobromine and paraxanthine in rat brain and serum

Pilar Parra, Anna Limon, Sergi Ferre, Teresa Guix, Francesc Jane

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    Abstract

    Caffeine and its metabolites theophylline, theobromine and paraxanthine have been determined in rat brain and serum samples by high-performance liquid chromatography with ultraviolet detection. The recovery, 85-103%, allowed quantification by external standard methods. The variability was found to be less than 3 and 7% for intra-day and inter-day assays, respectively. The detection limit, 1.57 ng of methylxanthines on column, allowed the determination of 62.5 ng/g or ml in biological material. Rats treated with 30 mg/kg caffeine (subcutaneously) were sacrificed at different times (1, 6, 12 and 24 h). Higher concentrations of methylxanthines (specially paraxanthine) were observed in the striatum than in the rest of the brain, and it was also observed that the clearance of methylxanthines was faster in serum than in brain structures. © 1991.
    Original languageEnglish
    Pages (from-to)185-190
    JournalJournal of Chromatography B: Biomedical Sciences and Applications
    Volume570
    Issue number1
    DOIs
    Publication statusPublished - 18 Sep 1991

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