TY - JOUR
T1 - High-fat diet induces metabolic changes and reduces oxidative stress in female mouse hearts
AU - Barba, Ignasi
AU - Miró-Casas, Elisabet
AU - Torrecilla, José L.
AU - Pladevall, Eulàlia
AU - Tejedor, Sergi
AU - Sebastián-Pérez, Rubén
AU - Ruiz-Meana, Marisol
AU - Berrendero, José R.
AU - Cuevas, Antonio
AU - García-Dorado, David
PY - 2017/2/1
Y1 - 2017/2/1
N2 - © 2016 Elsevier Inc. After an acute myocardial infarction, obese patients generally have a better prognosis than their leaner counterparts, known as the “obesity paradox”. In addition, female sex is associated with a lower risk of cardiac ischemic events and smaller infarct size compared to males. The objective of the present work was to study the metabolic phenotype and mitochondrial function associated to female sex and short-term high-fat diet. 1H NMR spectra of mice heart extracts were analysed by mRMR variable selection and linear discriminant analysis was used to evaluate metabolic changes. In separate experiments, O2 consumption and H2O2 production were measured from isolated mitochondria as well as serum oxidation susceptibility. Fingerprinting showed that male hearts contained more myo-inositol, taurine and glutamate than female hearts. HFD reduced the levels of creatine, taurine citrate and acetate. Profiling showed increased alanine and fumarate in HFD suggesting altered glycolitic and Krebs cycle pathways. Female mice contained less glucose than males. Female sex nor HFD altered mitochondria oxygen consumption but both conditions reduced the amount of H2O2 produced in an additive manner. Serum of females had lower oxidation susceptibility than serum from males but there were no differences associated with HFD. In conclusion, female sex and short-term HFD have an effect on the myocardial metabolic pattern and reduce the amount of H2O2 produced by mitochondria in an additive manner suggesting different mechanisms of action. This could explain, at least in part, the protection afforded by female sex and the “obesity paradox”.
AB - © 2016 Elsevier Inc. After an acute myocardial infarction, obese patients generally have a better prognosis than their leaner counterparts, known as the “obesity paradox”. In addition, female sex is associated with a lower risk of cardiac ischemic events and smaller infarct size compared to males. The objective of the present work was to study the metabolic phenotype and mitochondrial function associated to female sex and short-term high-fat diet. 1H NMR spectra of mice heart extracts were analysed by mRMR variable selection and linear discriminant analysis was used to evaluate metabolic changes. In separate experiments, O2 consumption and H2O2 production were measured from isolated mitochondria as well as serum oxidation susceptibility. Fingerprinting showed that male hearts contained more myo-inositol, taurine and glutamate than female hearts. HFD reduced the levels of creatine, taurine citrate and acetate. Profiling showed increased alanine and fumarate in HFD suggesting altered glycolitic and Krebs cycle pathways. Female mice contained less glucose than males. Female sex nor HFD altered mitochondria oxygen consumption but both conditions reduced the amount of H2O2 produced in an additive manner. Serum of females had lower oxidation susceptibility than serum from males but there were no differences associated with HFD. In conclusion, female sex and short-term HFD have an effect on the myocardial metabolic pattern and reduce the amount of H2O2 produced by mitochondria in an additive manner suggesting different mechanisms of action. This could explain, at least in part, the protection afforded by female sex and the “obesity paradox”.
KW - Antioxidant capacity
KW - Gender differences
KW - Metabolomics
KW - Mitochondrial function
KW - Myocardial metabolism
KW - NMR
KW - Obesity
U2 - 10.1016/j.jnutbio.2016.11.004
DO - 10.1016/j.jnutbio.2016.11.004
M3 - Article
VL - 40
SP - 187
EP - 193
JO - Journal of Nutritional Biochemistry
JF - Journal of Nutritional Biochemistry
SN - 0955-2863
ER -