Heptyl oleate synthesis as useful tool to discriminate between lipases, proteases and other hydrolases in crude preparations

Pablo Domı́nguez de Marı́a, Fernando Martı́nez-Alzamora, Sergio Pérez Moreno, Francisco Valero, M. Luisa Rúa, José M. Sánchez-Montero, José V. Sinisterra, Andrés R. Alcántara

    Research output: Contribution to journalArticleResearchpeer-review

    23 Citations (Scopus)

    Abstract

    Heptyl oleate synthesis is a good test to compare crude lipases. Being a reaction selective for lipases, esterases and proteases are not active in this esterification. The heptyl oleate synthesis has been carried out with crude lipases and other hydrolases (such as proteases and esterases) from different suppliers and origins, in order to explore the methodology scope. While the initial rate of esterification seems to be mainly dependent on the concentration of lipases in the sample tested, the final synthetic yield depends on the total amount of water in the reactor. The standard protocol to evaluate crude lipases is described. © 2002 Elsevier Science Inc. All rights reserved.
    Original languageEnglish
    Pages (from-to)283-288
    JournalEnzyme Microb. Technol.
    Volume31
    Issue number3
    DOIs
    Publication statusPublished - 2 Aug 2002

    Keywords

    • Esterases
    • Heptyl oleate synthesis
    • Hydrolysis
    • Lipases

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