Hepatic lipid metabolism changes in short- and long-term prehepatic portal hypertensive rats

Maria Angeles Aller, Alejandro Angulo, Fernando Sńchez-Patán, Ana Calderón, Jaime Arias, Elena Vara, Cruz García, Maria Paz Nava, Patri Vergara

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18 Citations (Scopus)

Abstract

Aim: To verify the impairment of the hepatic lipid metabolism in prehepatic portal hypertension. Methods: The concentrations of free fatty acids, diacylglycerol, triglycerides, and phospholipids were assayed by using D-[U-14C] glucose incorporation in the different lipid fractions and thin-layer chromatography and cholesterol was measured by spectrophotometry, in liver samples of Wistar rats with partial portal vein ligation at short- (1 mo) and long-term (1 year) (i.e. portal hypertensive rats) and the control rats. Results: In the portal hypertensive rats, liver phospholipid synthesis significantly decreased (7.42 ± 0.50 vs 4.70 ± 0.44 nCi/g protein; P < 0.01) and was associated with an increased synthesis of free fatty acids (2.08 ± 0.14 vs 3.36 ± 0.33 nCi/g protein; P < 0.05), diacylglycerol (1.93 ± 0.2 vs 2.26 ± 0.28 nCi /g protein), triglycerides (2.40 ± 0.30 vs 4.49 ± 0.15 nCi /g protein) and cholesterol (24.28 ± 2.12 vs 57.66 ± 3.26 mg/g protein; P < 0.01). Conclusion: Prehepatic portal hypertension in rats impairs the liver lipid metabolism. This impairment consists in an increase in lipid deposits (triglycerides, diacylglycerol and cholesterol) in the liver, accompanied by a decrease in phospholipid synthesi. © 2006 The WJG Press. All rights reserved.
Original languageEnglish
Pages (from-to)6828-6834
JournalWorld Journal of Gastroenterology
Volume12
Issue number42
DOIs
Publication statusPublished - 14 Nov 2006

Keywords

  • Cholesterol
  • Diacylglycerides
  • Free fatty acids
  • Phospholipids
  • Portal hypertension
  • Triglycerides

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